Only one study (Galindo-Barboza et al., 2011) has specifically examined the persistence of efficacy of COWP based on worm counts in sheep.

Recent data from goats managed under communal farming conditions suggest that egg counts are reduced two weeks, but not six weeks, after treatment with COWP (Spickett et al., 2012). However, no worm count data are available on the duration of efficacy of COWP in groups of goats subjected to similar levels of parasite exposure, nutrition and management. The present study therefore sought to examine the effect of COWP treatment in goats treated and removed from infective pasture at three different stages, namely at 7, 28 and 56 days post treatment. The use of animals for this experiment met the requirements CT99021 molecular weight of the Onderstepoort Veterinary Institute Animal Ethics Committee. A 0.67 ha pasture of star grass (Cynodon incompletus Nees) at Onderstepoort Veterinary Institute, Pretoria was utilized for the study in 2006–2007. In the spring of 2006, six months prior to the start of the actual experiment, the grass

was cut and fertilized. The pasture was irrigated through the spring and summer until the conclusion of the experiment in the following autumn if less than 25 mm rain fell during the previous week. Rainfall data were collected at Onderstepoort Veterinary Institute while temperature data were obtained from the Ibrutinib mw South African Weather Service for central Pretoria, which is approximately 16 km south of the Institute. Since the pasture had not been used for animal grazing for several years prior to the experiment, it was seeded with H. contortus larvae by grazing infected sheep on it. Initially, twenty indigenous the sheep were purchased from a commercial vendor, transported to Onderstepoort Veterinary Institute and maintained in concrete pens which were swept clean daily to preclude accidental nematode infection. The

animals were fed a commercial pelleted feed and lucerne (Medicago sativa) hay and the animals had free access to water. The sheep were dewormed with 10 mg/kg fenbendazole (Panacur BS®, Intervet South Africa) and 7.5 mg/kg levamisole (Tramisol®, Coopers, Afrivet Business Management, South Africa) daily for 5 days, followed by 0.3–0.5 mg/kg ivermectin (Ivomec Injection®, Merial South Africa) administered 6 days and 13 days after the combination treatment with fenbendazole and levamisole. Thirty-three days later, the animals were infected with 5000 third-stage larvae of a susceptible strain of H. contortus given as 1000 larvae per day for five days, as low-level, trickle dosing has been shown to be the optimal method for achieving establishment of parasites ( Barger et al., 1985 and Dobson et al., 1990).