15 EIPA also increases expression of the serine/arginine-rich (SR) splicing factor SRp20, which regulates exon 10 skipping in the tau transcript.15 EIPA also increased the expression level of alternatively spliced variants of ATP7B exon 12, suggesting splice-correction therapy could be used to treat patients with WD. Because Sotrastaurin cell line skipping exons 6, 7, 8, 12, and 13 produces in-frame ATP7B transcripts, it is important
to determine the function of these ATP7B variants to determine whether splice-correction therapy can be used for patients with deletions in or mutations on these exons. Mutation analysis of the ATP7B gene from patients with WD around the world revealed more than 380 disease-causing mutations, but only a few common mutations have been identified in specific populations. For example, a mutation in exon 14, His1069Glu, was predominantly detected in 17%-42% of North American, Greek, Polish,
Swedish, German, or British patients. In exon 18, another mutation hotspot, Gly1266Lys, has a 10% mutation rate among French and British patients.33 The most frequent mutation in exon 8, Leu708Pro, was found in the population of the Canary Islands, where it accounts for 50% of all mutations in the exon. For Asian populations in Korea, Japan, China, and Taiwan, Arg778 mutations in exon 8 account for more than 20% of all WD mutations. The Thr935Met in exon 12 has a mutation rate of 10% among Chinese patients. A mutation in exon 13, Selleck Dasatinib 2871delC, has a 15.9% mutation rate among Japanese patients.33 Thus, ATP7B mutations in Caucasian populations BCKDHA are common in exons 8 and 18, whereas mutations in Asian populations tend to occur in exons 8, 12, and 13. Because mutations on exons 8, 12, and 13 account for more than 50% of all WD mutations in Asian patients and exon 8 is a mutation hotspot in Caucasian
populations, splice-correction therapy may be a therapeutic option for WD, particularly for patients who cannot receive the standard penicillamine treatment. Acknowledgment: We thank Dr. Carmay Lim and Dr. Jim Sheu for critical review of this manuscript. Additional Supporting Information may be found in the online version of this article. “
“MicroRNAs (miRNAs) and methionine adenosyltransferase 1A (MAT1A) are dysregulated in hepatocellular carcinoma (HCC), and reduced MAT1A expression correlates with worse HCC prognosis. Expression of miR–664, miR–485–3p, and miR–495, potential regulatory miRNAs of MAT1A, is increased in HCC. Knockdown of these miRNAs individually in Hep3B and HepG2 cells induced MAT1A expression, reduced growth, and increased apoptosis, while combined knockdown exerted additional effects on all parameters. Subcutaneous and intraparenchymal injection of Hep3B cells stably overexpressing each of this trio of miRNAs promoted tumorigenesis and metastasis in mice.