42, df = 6, p = 0.76; Fig. 2). A similar disparity is evident for specific diversity-divergence categories P505-15 solubility dmso to cluster in a specific region even if only the most extreme samples that have the highest relative diversity or divergence in each species are included (χ 2 = 25.19, df = 18, p = 0.12). Table 3 Relative diversity-divergence patterns in different regions of the Baltic Sea indicated by the number of samples from each of the seven

species separately that fall into either of the four relative categories identified by Swatdipong et al. (2009), (i) higher diversity-higher divergence, (ii) higher GF120918 diversity-lower divergence, (iii) lower diversity-higher divergence, and (iv) lower diversity-lower divergence Diversity: Higher Higher Lower Lower   Divergence: Higher

Lower Higher Lower   Bothnian Bay 2 3 1 – 6 The Kvark 1 2 3 1 7 Bothnian Sea 1 5 1 1 8 Gulf of Finland – 3 4 – 7 Baltic Proper East – 1 4 1 6 Baltic Proper West 3 4 4 1 12 South Baltic 2 4 4 – 10   9 22 21 4 56 The different diversity-divergence categories do not favor any particular geographic region (χ 2 = 13.846, df = 18, p = 0.739). There is also a lack of tendency for high- or low-divergence samples from different species to occur in the same geographic region (χ 2 = 7.79, df = 6, p = 0.25). Similarly, samples with relatively high or low genetic diversity do not cluster

in any particular region (χ 2 = 3.41, df = 6, p = 0.75) Fig. 3 Association between geographic and genetic distance (isolation by distance, IBD). Correlation coefficients for line equation and significance many level of Mantel test (*0.05 > p > 0.01, *0.01 > p > 0.001, ***0.001 > p). Two Mantel tests were performed, one for the total material (all points, dotted line) and one for Baltic only samples (filled points, full line) Four of the species: Northern pike, whitefish, nine-spined stickleback and bladderwrack show significant pairwise differentiation between almost all samples (Table S2a–g). Although overall values of F ST are moderate in the three first species, the significant values imply limited gene flow among most sampling areas. We observe isolation by distance in both species of freshwater origin (pike and whitefish), but apart from that there are few similarities between these two species regarding location of PCI-32765 barriers and samples of high diversity or divergence. Isolation by distance was also present for herring when the Atlantic sample was included, but was not detectable in any other species in this study (Fig. 3).