7) 4 (10.5) 5
(9.4) Tetracycline (TET) 1 (6.7) 6 (15.8) 7 (13.2) Co-trimoxazole (COT) 14 (93.3) 5 (13.2) 19 (35.8) Chloramphenicol (CL) 2 (13.3) 2 (5.3) 4 (7.5) Amoxicillin-clavulanate (AMC) 15 (100) 16 (42.1) 31 (58.5) Ciprofloxacin (CIP) 1 (6.7) 0 (0) 1 (1.9) Pefloxacin (PEF) (0) 0 (0) 0 (0) PCR for the detection of antibiotic resistance genes Correlation between phenotypes and genotypic traits of resistance to the antibiotics was absolute. The aac(6′)-aph(2″) gene was VX-680 supplier detected in all the three isolates resistant to see more gentamicin while four out of the five erythromycin resistant isolates (2 S. epidermidis, 2 S. haemolyticus, 1 S. cohnii) were positive to erm(C). The remaining S. haemolyticus isolate had msr(A) gene. The tet(K) gene was detected in 6 (3 S. haemolyticus, 1 S. xylosus, 1 S. capitis, 1 S. cohnii) out of the 7 tetracycline resistant isolates while 4 (2 S. haemolyticus, 1 S. xylosus and 1 S. capitis) possessed the tet(M) gene. Three of the isolates (S. haemolyticus, S. xylosus and S. capitis) had both genes. All the fifteen oxacillin resistant isolates possess the mecA gene and were taken as MRCoNS. SCCmec typing SCCmec types were assigned for 13 of the mecA positive isolates (Table 2).
SCCmec type comprised of SCCmecI- ccrABβ2-α2 (4 isolates: 3 S. epidermidis, 1 S. warneri), SCCmecIVb- ccrABβ2-α3 (1 isolate: S. epidermidis), SCCmecIVd- ccrABβ2-α3 (8 isolates: 3 S. epidermidis, 2 S. xylosus, 1 S. saprophyticus, 1 S. warneri, 1 S. capitis). Two of the mecA positive isolates (S. epidermidis) were found to be untypable. Table 2 Phenotypes and genotypes of antibiotic resistance and SCC mec types Phenotype Genotype Strain selleck compound (Unique strain ID) PEN OXA GEN ERY TET COT CL CIP nuc mecA aac-aph erm(A) erm(B) erm(C) msrA tetM tetK SCCmec type ccr complex
S. capitis (SC01) R R S S S R S S – + – - – - – - – IVd ccrABβ2-α3 S. epidermidis (SE01) R R S S S R S S – + – - – - – - – I ccrABβ2-α2 S. epidermidis (SE02) R R S S S R S S – + – - – - – - – I S. epidermidis (SE03) R R S S S R S S – + – - – - – - – I S. epidermidis (SE04) R R S S S R S S – + – - – - – - – IVb ccrABβ2-α3 S. epidermidis (SE05) R R R S S R R R – + + – - – - – - IVd ccrABβ2-α3 S. epidermidis (SE06) R R S S S R S S – + – - – - – - – IVd ccrABβ2-α3 S. epidermidis (SE07) R R S S S R Dipeptidyl peptidase S S – + – - – - – - – IVd ccrABβ2-α3 S. epidermidis (SE08) R R S S S S S S – + – - – - – - – untypable Untypable S. epidermidis (SE09) R R S R S R S S – + – - – + – - – untypable Untypable S. saprophyticus (SS01) R R S S S R S S – + – - – - – - – IVd ccrABβ2-α3 S. warneri (SW01) R R S S S R S S – + – - – - – - – I S. warneri (SW02) R R S S S R S S – + – - – - – - – IVd ccrABβ2-α3 S. xylosus (SX01) R R S S R R R S – + – - – - – + + IVd ccrABβ2-α3 S. xylosus (SX02) R R S S S R S S – + – - – - – - – IVd ccrABβ2-α3 S. capitis (SC02) R S S S S S S S – - – - – - – - – S.