Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, Alatossava T: Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol 2000, 66:297–303.PubMedCrossRef 40. Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP: Detection of Lactobacillus, Pediococcus,

Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 2001, 67:2578–2585.PubMedCrossRef 41. Bassam selleck screening library BJ, Caetano-Anollés G, Gresshoff PM: Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 1991, 196:80–83.PubMedCrossRef 42. Heilig HGHJ, Zoetendal EG, Vaughan EE, Marteau P, Akkermans AD, de Vos WM: Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific

amplification of 16S ribosomal DNA. Appl Environ Microbiol 2002, 68:114–123.PubMedCrossRef 43. Kok RG, de Waal A, Schut F, Welling GW, RGFP966 Weenk G, Hellingwerf KJ: Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces. Appl Environ Microbiol 1996, 62:3668–3672.PubMed 44. Tilsala-Timisjärvi A, Alatossava T: Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR. Int J Food Microbiol 1997, 35:49–56.PubMedCrossRef 45. Zariffard MR, Saifuddin M, Sha BE, Spear Dapagliflozin GT: Detection of bacterial vaginosis-related organisms by real-time PCR for Lactobacilli, Gardnerella vaginalis and Mycoplasma hominis . FEMS Immunol Med Microbiol 2002, 34:277–281.PubMedCrossRef 46. Matsuki T, Watanabe K, Fujimoto J, Takada T, Tanaka R: Use of 16S rRNA gene-targeted group-specific primers for

real-time PCR analysis of predominant bacteria in human feces. Appl Environ Microbiol 2004, 70:7220–7228.PubMedCrossRef 47. Matsuki T, Watanabe K, Fujimoto J, Miyamoto Y, Takada T, Matsumoto K, Oyaizu H, Tanaka R: Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces. Appl Environ Microbiol 2002, 68:5445–5451.PubMedCrossRef 48. Rinttilä T, Kassinen A, Malinen E, Krogius L, Palva A: Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in Selleckchem PLX-4720 faecal samples by real-time PCR. J Appl Microbiol 2004, 97:1166–1177.PubMedCrossRef 49. Vignali DA: Multiplexed particle-based flow cytometric assays. J Immunol Methods 2000, 243:243–255.PubMedCrossRef Competing interests VSL Pharmaceuticals, Inc. is financing the article-processing charge. The authors declare that they have no other competing interests. Authors’ contributions BV performed the study design, analysis and interpretation of the data and the writing of the paper.