After 2 weeks of adaptation consuming a total mixed ration (TMR;

After 2 weeks of adaptation consuming a total mixed ration (TMR; 219 and 141 g/kg of crude protein (CP) and neutral detergent fibre (NDFom), respectively), lambs were weighed and distributed by BW into 4 pens of 8 lambs/pen in a completely randomized design with: Control group TMR; SB group TMR (as Control plus S. babylonica extract at 30 ml/d); LL group TMR (as Control plus L leucocephala extract at 30 ml/d); SBLL group TMR (as Control

plus 30 ml/d of S. babylonica and L leucocephala extracts in a (1:1, v:v) mixture) for a 63 days experiment. Weekly stock volumes (21 each) of the individual extracts as well as their eFT-508 clinical trial 1:1 mixture were prepared for daily administration. Extracts were orally administered before the 08:00 h feeding to each lamb. Feed intake was recorded daily and BW was recorded at MAPK inhibitor d 22 (P1), d 43 (P2) and d 63 (P3) of the experiment. Each experimental period ended with a digestibility measurement. Average daily gain (ADG), metabolizable protein (MP) intake and metabolizable energy (ME) intake were calculated. Plant secondary metabolites of total phenolics, saponins and the aqueous fraction were 50% lower in SB versus LL extracts. An extract x experimental period interaction occurred for intake of all nutrients (SB > LL = Control > SBLL, P<0.05). Intake

of DM, OM, CP and ADFom were lowest in P3 than other periods, whereas the highest nutrients intake were in P2 and P1 (P<0.05). Individual extracts improved (SB >

LL, P=0.05) the digestible DM, OM, EE and CP more than their 1:1 mixture compared with control or SBLL. Digestible DM was improved (P=0.03) by 5 and 3% with SB and LL extracts respectively, compared to control, whereas digestible CP was improved (P=0.005) by 7 and 5%, respectively, compared to control. Digestible ADFom was drastically lower in P1 and increased steadily in periods P2 and P3 (P<0.001). AZD9291 research buy Feed intake was not affected by extract addition although it was reduced from P1 and P2 to P3. Relative to control, addition of extracts increased (P=0.024) ADG while the other performance parameters (i.e., MP intake, ME intake) had a tendency (P=0.05) to improve with extract addition. ADG was improved by about 22 and 21% with SB and LL extracts respectively, while their mixture (i.e., SBLL) was 2% compared to control (i.e., SB = LL > Control = SBLL, P=0.024). MP and ME intakes were decreased (P<0.0001) from P1 and P2 to P3. Results show that daily administration of individual extracts of S. babylonica and L leucocephala improved lamb performance by 22 and 21%, but that a mixture of both had a much lower benefit. (C) 2011 Elsevier B.V. All rights reserved.

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