\n\nConclusion: The 4-year BDFS after HIFU and HIFU + TURP is comparable to that of the standard therapies. The erectile function is sustainably negatively influenced whereas postoperative micturition and quality of life were not affected by HIFU or HIFU + TURP. These results are strongly limited by the low patient count and the short follow-up period and require validation in prospective multicentre studies with higher number of cases.”
“The determination of protein crystal structures

is hampered by the need for macroscopic crystals. X-ray free-electron lasers (FELs) S63845 provide extremely intense pulses of femtosecond duration, which allow data collection from nanometre- to micrometre-sized crystals(1-4) in a ‘diffraction-before-destruction’ approach. So far, all protein structure determinations carried out using FELs have been based on previous knowledge of related,

known structures(1-5). Here we show that X-ray FEL data can be used for selleck kinase inhibitor de novo protein structure determination, that is, without previous knowledge about the structure. Using the emerging technique of serial femtosecond crystallography(1-4,6), we performed single-wavelength anomalous scattering measurements on microcrystals of the well-established model system lysozyme, in complex with a lanthanide compound. Using Monte-Carlo integration(6,7), we obtained high-quality diffraction intensities from which experimental phases

could be determined, resulting in an experimental electron density map good enough for automated building of the protein structure. This demonstrates the feasibility HDAC inhibitor of determining novel protein structures using FELs. We anticipate that serial femtosecond crystallography will become an important tool for the structure determination of proteins that are difficult to crystallize, such as membrane proteins(1,2,8).”
“Background: Despite the proclivity and proliferation of blogs on the Internet, the use of blogs at medical institutions is not well documented. Aims: In examining the structured stories that medical students share with the digital community, we may better understand how students use institutional blogs to discuss their medical school experiences while maintaining their role as a medical student ambassador for the program. Methods: We conducted a case study to analyze the stories within 309 medical student blogs from one medical institution in the United States. Results: In an attempt to communicate their experiences to different benefactors, student bloggers engaged in structured and personal storytelling. Structured stories offered medical school advice to prospective students, while personal stories embodied features of a personal diary where students recounted significant milestones, talked about personal relationships and engaged in emotional reflection and disclosure.