Cun Daily renal clearance rate of urea Fig. 2 The relationship between the amount of urinary excreted YM155 cost total protein and albumin (a), and the relationships between the amount of urinary excreted Klotho and urinary total protein (b) and that of urinary albumin (c) among PD patients. Note that there was a significant

association between the amount of urinary total protein and albumin The soluble Klotho excretion in the dialysate was also determined. Although there was one patient with a negative finding for Klotho, Klotho was detectable by ELISA in the dialysate of the remaining thirty-five patients. The amount of peritoneal excreted soluble Klotho in the 24-h dialysate collections ranged from 21.7 to 323.5 ng/day (median 113.7 ng/day; IR 76.9–155.1). There were significant correlations between the amount of peritoneal Klotho excretion and those of total protein and albumin (Fig. 3a, b). We also confirmed that a significant relationship existed between the amount of total protein and that of albumin in the dialysate (Fig. 3c). An IB analysis demonstrated the presence of soluble Klotho in the dialysate Saracatinib molecular weight derived from the patient with the highest Klotho concentration (Fig. 3d). We failed to confirm a statistically significant relationship

between the amount of peritoneal soluble Klotho and the single-day peritoneal KT/V (r = −0.163, Selleckchem BIBF 1120 p = 0.35). Fig. 3 The relationships between

the amount of peritoneal Klotho excretion and those of total protein (a) and albumin (b) in the dialysate, and the relationship between the amount of peritoneal total protein and that of albumin (c). d The presence of soluble Klotho was demonstrated by immuno-blotting (IB) with KM2076 in the peritoneal dialysate samples with the highest Klotho concentration, of 122.7 pg/ml (lane 1), and the second-highest Klotho concentration, below of 54.4 pg/ml (lane 2). In the sample from the patient with a Klotho concentration of 21.1 pg/ml, no band was detectable (lane 3). Note that the densities of the bands seemed to correspond to the soluble Klotho concentrations Discussion The present study has clearly demonstrated, for the first time, that the urinary excreted soluble Klotho, but not the serum Klotho concentration, is positively correlated with approximations of the residual renal function, including the urine output, urinary Ccr, and the average urinary Ccr + Cun [13, 14], among patients undergoing PD treatment. The urine output among patients with chronic renal failure may be variable, ranging from oliguria to normal or even above normal levels, because it is determined not only by the glomerular filtration rate (GFR), but also by numerous pathophysiological mechanisms, such as changes in the rate of tubular reabsorption [15].