“
“Lipoxygenase enzymatic pathway is

a widely studied mechanism in the plant kingdom. Combined actions of three enzymes: lipase, lipoxygenase (LOX) and hydroperoxide lyase (HPL) convert lipidic substrates such as NVP-BSK805 nmr C(18:2) and C(18:3) fatty acids into short chain volatiles. These reactions, triggered by cell membrane disruptions, produce compounds known as Green Leaf Volatiles (GLVs) which are C(6) or C(9)-aldehydes and alcohols. These GLVs are commonly used as flavors to confer a fresh green odor of vegetable to food products. Therefore, competitive biocatalytic productions have been developed to meet the high demand in these natural flavors. Vegetable oils, chosen for their lipidic acid profile, are converted by soybean LOX and plant HPL into natural GLVs. However this second step of the bioconversion presents low yield due to the HPL instability and the inhibition by its substrate. This paper will shortly describe the different enzymes involved in this bioconversion with regards to their chemical and enzymatic properties. Biotechnological techniques to enhance their production potentialities will be discussed along with their implication in a complete bioprocess, from the lipid substrate to the corresponding aldehydic or alcoholic flavors.”
“Methanolic extracts Dinaciclib of Solanum nigrum

(leaves and seeds of both black and red varieties), Elettaria cardamomum Cuscuta reflexa and Cinnamomum camphora were tested in vitro for their antibacterial and antifungal

activities. Antibacterial study performed against six bacteria viz., Escherichia coli, Citrobacter, Shigella flexenari, Staphylococcus Blasticidin S aureus, Pseudomonas aeruginosa and Yersinia aldovae indicated that investigated plants have potent activity against all microorganisms. The antifungal activity of these extracts was performed against six fungi, viz., Saccharomyces cereviciae, Aspergillus parasiticus, Trichophyton rubrum, Macrophomina, Fusarium solani and Candida albicans. The extracts showed moderate as well as significant activity against different fungal strains.”
“Wnts comprise a family of lipid-modified, secreted signaling proteins that control embryogenesis, as well as tissue homeostasis in adults. Post-translational attachment of palmitoleate (C16:1) to a conserved Ser in Wnt proteins is catalyzed by Porcupine (Porcn), a member of the membrane bound O-acyltransferase (MBOAT) family, and is required for Wnt secretion and signaling. Moreover, genetic alterations in the PORCN gene lead to focal dermal hypoplasia, an X-linked developmental disorder. Despite its physiological importance, the biochemical mechanism governing Wnt acylation by Porcn is poorly understood. Here, we use a cell-based fatty acylation assay that is a direct readout of Porcn acyltransferase activity to perform structure-function analysis of highly conserved residues in Porcn and Wnt3a. In total, 16-point mutations in Porcn and 13 mutations in Wnt3a were generated and analyzed.