Local and systemic inflammation ensued without any apparent trigger or autoimmune aetiology (see accompanying Viewpoint by Meng and Strober 6). Characterization of the causative mutations in NLRP3 underlying CAPS has had a direct impact on the clinic, leading to successful therapy of CAPS in the form of IL-1 blockade (Anakinra) 7–11. Interestingly, gout, an inflammatory condition caused by chronic activation of the NLRP3 inflammasome in response to tissue-derived monosodium urate crystals 12, also seems to benefit from IL-1 blockade therapy 13. Nonetheless despite this significant progress, there remain a significant number of patients with recurrent fever syndromes who

respond to IL-1 inhibition but with no demonstrable NLRP3 mutations. A check details recent study has identified mutations in NLRP12 that cause hereditary periodic fever syndromes 14, demonstrating a crucial regulatory role of NLRP12 in the inflammasome pathway and reinforcing the possibility of as yet undiscovered disease-causing mutations in genes along the inflammasome-IL-1β axis. Other well-characterized inflammasomopathies include familial Mediterranean fever 15), pyogenic CT99021 ic50 arthritis with pyoderma gangrenosum and acne syndrome 16), recurrent hydatidiform mole 17, 18 and vitiligo 19, 20. Positional cloning techniques mapped the causative mutations in familial Mediterranean fever to the MEFV gene encoding pyrin, to

the gene encoding PSTPIP1 in pyogenic arthritis with pyoderma gangrenosum and acne

syndrome and to NLRP7 in recurrent hydatidiform mole, whereas SNP association analyses identified NLRP1 as a risk factor for vitiligo and recently linked NLRP3 to CD 21 (see below). The precise mechanisms by which these mutations or SNP lead to disease are not clearly understood (Table 1). For instance, it is unclear whether pyrin is a negative or positive regulator of IL-1β release. It has been suggested that through its direct interaction with the inflammasome adaptor ASC, pyrin inhibits IL-1β activation by competing with caspase-1 and NLRP3 for ASC 15, 22, 23. Paradoxically, Phosphatidylinositol diacylglycerol-lyase pyrin has also been reported to assemble an ASC pyroptosome that activates caspase-1 and induces pyroptosis and IL-1β release 24, 25. PSTPIP1 interacts with pyrin and mutations in PSTPIP1 were shown to enhance this binding, modulating pyrin functions 16, 26. NLRP7 has been proposed as a negative regulator of IL-1β production 27, yet it remains to be determined whether the NLRP7 mutations inactivate this function. Our understanding of how NLR-coupled inflammasomes function in vivo in both normal and disease states will undoubtedly continue to advance over the next few years. Although excessive production of IL-1β by caspase-1 is harmful, as discussed above, its regulated production is critical for the control of pathogenic infections and of severe sepsis.