Material examined:

THAILAND, Chiang Rai Province., Muang District, Thasud Sub District, on dead twig of Eucalyptus sp., 8 August 2011, M. Doilom (MFLU 12–0760), living culture MFLUCC 11–0508. Leptoguignardia E. Müll., Sydowia 9: 216 (1955) selleckchem MycoBank: MB2777 Hemibiotrophic or saprobic on petioles. Ascostromata black, scattered, clustered or fusing in groups of 2–3, initially immersed, becoming erumpent but still under host tissue, ovoid to globose, coriaceous. Papilla central, ostiole with a pore. Pseudoparaphyses sparse, hyphae-like, not commonly observed in herbarium material. Peridium comprising small heavily pigmented thick-walled cells of textura angularis, Asci 8–spored, bitunicate, Selleck Belinostat fissitunicate, with a short blunt pedicel, ocular chamber not clear. Ascospores hyaline, 2–septate, fusiform, asymmetrical, central cells widest, ends cells longer

and tapering, smooth-walled. Asexual “Dothichiza”-like morph forming on same tissue. Pycnidia Selleck Semaxanib black, scattered, or fusing in groups or with locules, immersed, becoming erumpent, but still under host tissue, ovoid, coriaceous, scattered amongst locules. Conidiogenous cells hyaline, cylindrical, holoblastic. Conidia hyaline, 1–septate, septum nearer to apex, slightly constricted, ovoid with round ends. Notes: Leptoguignardia was introduced by Müller (1955) and is monotypic represented by the generic type Leptoguignardia onobrychidis E. Müll. The taxon occurs on dead petioles of Onobrychidis montanae in France. There is no sequence data available for this species, but based on its ascomata and ascial Prostatic acid phosphatase characters, it fits well into Botryosphaeriaceae, although new collections are required to confirm this. Generic type: Leptoguignardia onobrychidis E. Müll. Leptoguignardia onobrychidis E. Müll., Sydowia 9: 217 (1955) MycoBank: MB299536 (Figs. 18 and 19) Fig. 18 Leptoguignardia onobrychidis (Myc 2232, holotype) a–c Habit and appearance

of ascostromata on host substrate. d–e Section trough ascostromata showing developing of asci. f–i Asci. j–k Ascospores. Scale bars: d–f = 50 μm, g–k = 10 μm Fig. 19 Asexual morph of Leptoguignardia onobrychidis (Myc 2232, holotype) a–c Habit and appearance of conidiomata on host substrate. d–f Section through pycnidia. g Conidiogenous cells. h–i Conidia. Scale bars: d–f = 50 μm, g-h = 10 μm Hemibiotrophic or saprobic on petioles. Ascostromata 100–110 μm high × 170–180 μm diam., black, scattered, clustered or fusing in groups of 2–3, initially immersed, becoming erumpent but still under host tissue, ovoid to globose, coriaceous. Papilla central, ostiole with a pore opening, 38–40 μm long.