On the other hand, proSP CWT, but rarely proSP CI73T, colocalized

On the other hand, proSP CWT, but rarely proSP CI73T, colocalized www.selleckchem.com/products/MDV3100.html with syntaxin 2, a SNARE protein involved in the secretion of lung surfactant, found in the plasma membrane and lamellar bodies of AECII. Interestingly, our data propose the influence of hydroxychloroquine and methylprednisolone on localization and routing of proSP CWT moving it toward early endosomal vesicles. On the other hand, methylprednisolone showed the capacity to partially correct the mislocalization routing defect of proSP CI73T. The expression of mutated proteins frequently results in elevated cell stress. This has been shown for the BRI CHOS domain SP C mutations L188Q and exon4. We found that the constitutive expression of SP CI73T moderately increased cell lethality under nor mal growth conditions, maybe as a result of the ability of the cellular system to adapt to the pre sence of stress, as reported in.

The additional exo genous stress, imposed in our experiments by exposure to pharmaceuticals used in ILD therapy, might shift this balance out of the tolerable range. Treatment of the cells with azathioprine drug almost doubled the number of dying I73T mutant cells compared to WT. This aggravation was much less pronounced in the presence of methylprednisolone, hydroxychloroquine or cyclophosphamide. Intracellular stress is in part handled by endogenous chaperones. Still without pharmacological boost, such cytoprotective mechanisms may not always be sufficient to normalize the cell function and maintain production of the bioactive surfactant with a normal lipid protein composition.

We determined the change in expression of the four important chaperones under the influence of the same ILD drugs. We found that the influence of azathioprine on the chaperones was almost the same in proSP CWT and proSP CI73T expressing cells, leaving no protection for additional stress, being a potent stress factor per se. In contrast, hydroxychloro quine treatment led to an 81% increase in HSP90, and 75% increase in calreticulin expression in I73T mutant cells over WT cells, thereby possibly protect ing the cells against the additional stress and enhancing the ER folding capacity. HSP90 seemed to be targeted by all tested pharmaceuticals, while calnexin levels were refractory to stimulation. Treatment with the four drugs did not change the pattern of the proSP C processing bands observed in the immunoblots in Figure 1A.

The lipid composition of the stable MLE 12 cells was similar to that previously described in human foetal AECII, especially with regard to PC composition. In the SP CI73T expressing cells we found a pronounced drop of total cellular PC, whereas LPC was increased. It is known that PC is degraded to LPC by an intrinsic phospholipase A2 like activity, and that LPC Batimastat is toxic to various cells. Increased LPC may therefore be a result of increased phospholipase activity due to the pre sence of mutated SP C.

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