Our recent meta-analysis of the predictive ability of GCN indicat

Our recent meta-analysis of the predictive ability of GCN indicated that it is a fairly good biomarker for response [14], however, only in non-Asian patient populations was it shown to be predictive www.selleckchem.com/products/i-bet151-gsk1210151a.html of improved PFS and OS, albeit from a limited number of studies most of which were not designed to investigate the particular biomarker [15]. Our data correlates with these previous data sets but does not assist greatly in understanding the differences seen between “Asian” and “non-Asian” studies. Regarding IHC expression of EGFR, this was found positive in 16% of the

cases tested and no correlation with clinical outcome was demonstrated. The IHC expression of EGFR protein varies across several studies and as such, has been an inconsistent predictor of response to EGFR inhibitors. In a retrospective analysis SB202190 supplier of tumor biopsy samples from patients treated in the BR.21 trial, 57% were found to over-express EGFR by IHC. Response to EGFR agonists was found higher among patients expressing EGFR, though the difference was statistically insignificant. Furthermore, EGFR protein status was not an independent predictor of OS in this study. In opposition, in the ISEL trial, patients with EGFR expressing tumors, as detected by IHC,

had significantly longer OS than patients with EGFR negative tumors. A combination of IHC and FISH status may be an effective predictor of responsiveness to EGFR TKIs, however, in our study this was not feasible due to the Selleck Abiraterone small number of cases for EGFR FISH and IHC. It has been demonstrated that somatic mutations in the EGFR TK domain are associated with responsiveness to EGFR TKIs [14]. We found that patients harboring EGFR mutations in exon 19/21 had a significantly better DCR as compared with those with no detectable mutations. These patients had also a longer PFS. Data from the INTEREST trial also showed that EGFR mutation was a predictive marker of prolonged PFS. More recently, the phase III IPASS study that randomized 1,217 patients to gefitinib versus carboplatin plus paclitaxel indicated the superior benefit obtained with gefitinib restricted to the EGFR mutation

positive population. Several subsequent studies support this data [32, 33]. Although buy PLX-4720 treatment with EGFR TKIs provides clinical benefit to some patients, many are primarily resistant to treatment. Furthermore, virtually all patients with an initial response to TKIs, even in the presence of activating sensitizing mutations, eventually relapse and demonstrate TKI resistance. Multiple underlying mechanisms of resistance have been described, including EGFR mutations, the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) pathway, MET amplification, and KRAS mutations [18]. Whereas activating mutations in the EGFR TK domain are associated with greater sensitivity to TKIs, some mutations are associated with resistance.

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