“
“Streptococcus pyogenes causes diseases as pharyngitis, impetigo, streptococcal toxic shock syndrome and inhibitors necrotizing fasciitis. Rheumatic fever (RF), acute streptococcal glomerulonephritis and rheumatic heart disease (RHD) are non-suppurative autoimmune post-streptococcal sequelae that arise from a delayed immune response to infection in genetically predisposed individuals [1]. Several markers are described as risk factors for RF/RHD, including HLA-DR7,

the allele most commonly associated with RHD in Brazil and other countries [2]. FGFR inhibitor According to the World Health Organization (WHO), S. pyogenes is responsible for 15–20% of bacterial pharyngitis cases, which primarily affect 5- to 18-year-old individuals [3]. The incidence of bacterial pharyngitis varies among countries, and even within the same country, there are variations in different regions due to age, socioeconomic and environmental factors and quality of health services [4] and [5]. The M protein has been described as the major bacterial antigen [6]. The protein consists of two polypeptide chains in an alpha double helix coiled-coil that forms fibrils extending up to 60 nm away from the bacterial surface. It is approximately 450 amino acids long

and is divided into tandem repeat blocks distributed over four regions (A, B, C and D). The N-terminal portion (regions A and B) is polymorphic and differences within the first 150 amino acid residues of the A region allow for the classification of different serotypes [7] and [8]. The C-terminal portion (regions C and D) is highly conserved, responsible for binding the bacteria to the oropharynx Quizartinib concentration mucosa and has antiphagocytic properties [6] and [7]. RF/RHD pathogenesis is related to the production of autoantibodies and autoreactive T cells that recognize and cross-react with epitopes from both the M protein and human heart tissue by molecular mimicry [9] and [10] and it was demonstrated by analyzing the T cell repertoire that infiltrated cardiac tissue and led to damage in RHD

[11]. M1 is the most common strain worldwide and, due to its high virulence, secondly is involved in invasive and non-invasive infections in several countries [12] and [13]. There is a large diversity of strains in Brazil. The most prevalent strains found in a sample from Sao Paulo city were the M1, M6, M12, M22, M77 and M87 compatible with those found in the rich districts from Salvador [5] and [14]. These M-types are also predominant in most of the world western countries [15]. Besides that, there is a much higher diversity of M-types in the poor districts from Salvador and Brasilia typically found in low incomes regions [5] and [16]. The classification of strains according to their tissue tropism for throat (A–C pattern), skin (D pattern) or both (E pattern) is based on the organization of emm and emm-like genes located in the mga locus within S. pyogenes genome and constitute the base for emm pattern genotyping [17] and [18].