The bacterium uses the pLcr plasmid-encoded type III secretion system to deliver virulence factors into host cells. Delivery requires ATP hydrolysis by the YscN ATPase

encoded by the yscN gene also on pLcr. A yscN mutant was constructed in the fully virulent CO92 strain containing a nonpolar, in-frame internal deletion within the gene. We demonstrate that CO92 with a yscN mutation was not able to secrete the LcrV protein (V-Antigen) and attenuated in a subcutaneous model of plague demonstrating that the YscN ATPase was essential for virulence. However, if the yscN mutant was complemented with a functional yscN gene in trans, virulence was restored. To evaluate the mutant as a live vaccine, Swiss–Webster mice were vaccinated twice with the ΔyscN mutant at varying doses and were protected against Tanespimycin bubonic plague in a dose-dependent manner. Antibodies to F1 capsule but not to LcrV were detected in sera from the vaccinated mice. These preliminary results suggest a proof-of-concept for an attenuated, genetically engineered, live vaccine effective against bubonic plague. Yersinia pestis is a zoonotic bacterial agent responsible for bubonic and www.selleckchem.com/products/H-89-dihydrochloride.html pneumonic plague, diseases which are transmitted through fleabites and aerosols, respectively (Perry & Fetherston,

1997). The bacterium uses a sophisticated virulence factor delivery system, the type III secretion system (T3SS), that is composed of the Ysc injectisome which secretes proteins referred to as Yops (Yersinia outer proteins) into host cells. The proteins for the T3SS are encoded by genes on the pCD1/pLcr plasmid (Cornelis et al., 1989; Straley, 1991). One of the Yops, LcrV, has various roles. It is surface-exposed prior to interacting with host cells, required for translocation of the effector Yops, and has some role

in Yop regulation (Nilles et al., 1998; Sarker et al., 1998a, b; Pettersson et al., 1999). Also, LcrV is highly antigenic and able to provide protection against plague challenges in animal models of disease (Une & Brubaker, 1984; Motin et al., 1994; Roggenkamp et al., 1997). While the delivery of some Protein kinase N1 Yops may require chaperones for secretion, other Yops do not. Yop delivery also requires cell-to-cell contact (Rosqvist et al., 1994), but the identity of the human receptor for Y. pestis is not known. A Y. pestis T3SS-specific ATPase, designated YscN and also encoded on pCD1/pLcr, removes chaperones from the Yops before translocation into mammalian hosts (Payne & Straley, 1998, 1999). The process requires ATP hydrolysis, but the details of transport are unknown (Akeda & Galan, 2005). It has been hypothesized that the energy for the translocation may be generated by a proton gradient (Paul et al., 2008); however, this hypothesis remains controversial (Galan, 2008). The YscN protein is the only ATPase required for chaperone removal and possibly for the translocation through the pore.