The present study was aimed at evaluating the diagnostic value of the aptamers in non-hepatic malignant tumors (NHMTs). Methods: Serum samples of NHMTs and benign diseases collected from hospitalized patients and normal serum samples from physical examination subjects were incubated with aptamers. The binding degree of aptamer to serum was determined by polyacrylamide gel electrophoresis and gray value analysis. EX 527 cost A diagnostic mathematical model was established by multivariate logistic

regression with the variables of gray value and its diagnostic value for NHMTs was evaluated. Results: NHMTs included esophageal cancer, gastric cancer, colorectal cancer, pancreatic cancer, lung cancer, kidney cancer, bladder cancer, prostate cancer, ovarian cancer, breast cancer, cervical cancer, osteosarcoma, head and neck cancer and lymphoma. Benign diseases included benign liver diseases (cirrhosis and hepatitis B) and benign non-liver diseases. The diagnostic value of aptamers for NHMTs was showed in Table 1

below. The aptamers being also valuable in the diagnosis of Staurosporine price non-hepatic malignant tumors suggest that targets of the aptamers may be common in hepatic and non-hepatic cancers. Conclusion: The aptamers against hepatic carcinoma serum are also valuable in the diagnosis of non-hepatic malignant tumors. Key Word(s): 1. Aptamer; 2. Hepatic carcinoma; 3. diagnosis; 4. malignant tumors; Table 1 The diagnostic performance of aptamers against hepatic carcinoma sera in non hepatic malignant tumors Aptamers and groups AUC SEN (%) SPE (%) ACC (%) PPV (%) NPV (%) PLR NLR NHMT: non-hepatic malignant until tumor; BLD: benign liver disease; NLBD: non-liver benign disease; SEN: sensitivity; SPEL: specificity; ACC: accuracy; PPV: positive predictive value; NPV: negative predictive value; PLR: positive

likelihood; NLR: negative likelihood ratio. Presenting Author: TING WANG Additional Authors: GUO-FENG XU, KUN-HE ZHANG, QIN ZENG, XUAN LI, QIN-SI WAN, HONG-LI ZHANG, XUAN ZHU, NONG-HUA LV Corresponding Author: KUN-HE ZHANG Affiliations: the First Affiliated Hospital of Nanchang University; Jiangxi Institute of Gastroenterology & Hepatology Objective: Aptamers are artificial nucleic acid ligands capable of binding to targets with high specificity and affinity. We previously selected a group of aptamers using pooled primary hepatic carcinoma (PHC) serum as target by SELEX (systematic evolution of ligands by exponential enrichment). The purpose of this study was to compare the diagnostic value of aptamers with alpha-fetoprotein (AFP) in malignant and benign liver diastases. Methods: The diagnostic value of 12 aptamers for malignant and benigh liver diastases was evaluated by use of polyacrylamide gel electrophoresis and gray analysis that we developed previously.