The results showed that the method proposed by the CEN/TC/WG6/TAG4 group was 100% specific since it was able to detect all samples tested. The commercialized
kits evaluated failed MK-8776 solubility dmso to detect a vast majority of NoV GI strains. Additionally the Generon kit did not succeed to detect strains from GII.3, GII.5, GII.6, GII.7, GII.8, GII.12 and GII.17. In addition, the detection limit using the most prevalent strain, NoV GII.4, was 2.5 PCRU per reaction using both commercial kits. Despite this good sensitivity for NoV GII.4 detection it is concluded that both commercial assays are not suitable for the detection and quantitation of most NOV subtypes. Therefore the method proposed by the CEN/TC/WG6/TAG4 group is recommended for epidemiological studies and outbreaks investigations. (C) 2010 Elsevier B.V. All rights reserved.”
“Effects of chronic restraint stress on the taste responses to five basic taste qualities were investigated electrophysiologically in the rat chorda tympani In addition the mRNA expression for T1R3 the common G-protein-coupled receptor (GPCR) for sweet and umami tastes was studied quantitatively by RT-PCR after such stress Rats were restrained in a small cylindrical restrainer made of steel wire for 8 h daily for 14 successive days The integrated responses to sweet and umami tastes as recorded from the chorda tympani were
significantly suppressed after such stress but the other three basic taste responses were unaffected Expression of T1R3 mRNA selleck products in the fungiform papillae Selleckchem Nutlin 3a as estimated by RT-PCR was slightly reduced by the stress and a quantitative real time RT-PCR study revealed a significant suppression of T1R3 mRNA expression in the stress group These results suggest that the observed stress-Induced changes in taste sensation could be caused by a peripheral disorder of the transduction mechanism in taste-receptor cells involving in particular a stress-induced inhibition of T1R3 expression (C) 2010 Elsevier Ireland Ltd All rights reserved”
“An improved bacmid technology for
cloning complete genomes of large dsDNA viruses with circular genomes has been developed and tested. The system, termed EZ::BAC, is based on Escherichia coli F factor replicon, a chloramphenicol resistant marker gene with the mosaic ends recognized specifically by the transposase of the Tn5. In vitro transposition was carried out for the baculovirus shuttle vector pMON14272 (136 kb) and the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome (134 kb) as target DNAs. Transposon EZ::BAC was inserted randomly into the target DNAs, leading to 9 bp duplication of the flanking end at the insertion site. One of the obtained AcMNPV::BACs replicated in Sf21 cells after transfection. The random in vitro generation of viral bacmids using EZ::BAC facilitates the host-independent propagation of intact and functional viral genomes in E.