Methods: Orthotopic vascularized right lung transplantations using cuff techniques were performed in syngeneic and allogeneic strain combinations. Grafts were assessed histologically or functionally by measuring arterial blood gases from 7 to 28 days after transplantation. In a parallel set of experiments, syngeneic and immunosuppressed allogeneic hosts underwent a left pneumonectomy 5-Fluoracil 2 weeks after right lung transplantation, with

assessment of graft function 1 week later.

Results: We performed 40 right lung transplantations, with a survival rate of 87.5%. Syngeneic grafts remain free of inflammation as far as 28 days after transplantation. On day 7, arterial oxygen levels in syngeneic recipients (481 +/- 90 mm Hg) are equivalent to those in naive mice (503 +/- 59 mm Hg) after left hilar occlusion. Alternatively, allogeneic grafts develop histologic evidence of acute rejection, and arterial oxygen levels are significantly decreased after left hilar clamping (53.3 +/- 10.3 mm Hg). Both syngeneic and

immunosuppressed allogeneic right lung recipients tolerate a left pneumonectomy.

Conclusions: Right lung transplantation followed by left pneumonectomy represents the first survival model of vascularized lung transplantation in the mouse and will therefore allow for the design of novel selleck chemicals studies in experimental lung transplantation. (J Thorac Cardiovasc Surg 2010;139:1637-43)”
“Neuroinflammation mediated by microglia has been implicated in neurodegenerative diseases. unless Suppression of microglial activation may therefore contribute to neuronal cell survival.

Chrysin is present in honey and propolis and in low concentrations in fruits, vegetables, and certain beverages. It has been reported that chrysin has potent anti-inflammation, anti-cancer, and anti-oxidation properties. In the present study, we investigated the effects of chrysin on the production of proinflammatory mediators in lipopolysaccharide (LPS)-stimulated microglia. Chrysin significantly inhibited the release of nitric oxide (NO) and proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) The expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) were also significantly inhibited by chrysin. Furthermore, chrysin inhibited the activations of c-Jun N-terminal kinase (JNK) and nuclear factor-kappa B (NF-kappa B), which are signaling molecules involved in neuroinflammation. These results suggest that chrysin may act as a potential therapeutic agent for various neurodegenerative diseases involving neuroinflammation. (C) 2010 Elsevier Ireland Ltd. All rights reserved.”
“Objectives: Plasminogen activator inhibitor 1 is the primary regulator of urokinase plasminogen activator and tissue plasminogen activator. Plasminogen activator inhibitor 1 is essential in the control of the thrombotic/fibrinolytic balance and is a marker of endothelial cell injury.