Results: Initially we performed transgastric endoscopic cholecyst

Results: Initially we performed transgastric endoscopic cholecystectomy in porcine models. Next we progressed to perform surgery with endoscopic accessories with endoscopy placed in the umbilicus, performed large human series of appendectomy and cholecystectomy. After gaining good experience of performing trans-umbilical endoscopic appendectomy and cholecystectomy, we advanced to vaginal route and performed large series of appendectomy and cholecystectomy. During this process, if difficulty was encountered it was converted to standard laparoscopy without learn more any added morbidity. Finally we proceeded

to transgastric cholecystectomy and appendectomy, at completion requires standard laparoscopic gastrotomy closure until we get a good tool to close the gastrostomy by endoscope. TRANSUMBILICAL ACCESS – Number of cases = 20 (12 appendectomies and 8 cholecystectomies) Age group = 27 ? 52 years Duration of hospital stay = 2 ? 4 days Operative time = 30 ? 110 minutes TRANSVAGINAL ACCESS – Number of cases = 80 (55 appendectomies and 25 cholecystectomies) Age group = 32 to 58 years Duration of hospital stay = 2 to 4 days Operative time = 35 to 110 minutes TRANSGSTRIC ACCESS Number Ruxolitinib of cases = 5 (3 appendectomies and 2 cholecystectomies) Age group 27 to 44 years Duration of hospital stay = 4 to 6 days Operative time = 90 to 135 minutes Conclusion: Since NOTES being

a complex procedure needing both laparoscopic surgical experience and therapeutic endoscopy, this method of step by step learning approach in performing this endoscopic surgical procedures is safe without any morbidity associated with new techniques. Key Word(s): 1. NOTES; 2. Transumbilical; 3. Transvaginal; 4. Transgastric; Presenting Author: ENQIANG MCE公司 LINGHU Additional Authors: ZHICHU QIN, ZHI QUN LI, YOU ZHANG, MAN MENG Corresponding Author: ENQIANG LINGHU Affiliations: Department of Gastroenterology and Hepatology, the PLA General Hospital Objective: Peroral endoscopic myotomy (POEM) is a new treatment for achalasia. The aim of our study was to investigate the healing

of esophageal tunnel incision and the falling of titanium clips after POEM. Methods: The time of esophageal tunnel incision healing and titanium clips falling of 30 patients with achalasia using PPI (proton pump inhibitors) for 1 month after POEM were retrospectively analyzed between May to December 2012, The follow-up time of endoscopic examination is 1 weeks, 2 weeks, 1months and 3 months. Results: 232 titanium clips had been used, the rate of all titanium clips falling off is 16.8% (39/232), 41.4% (96/232), 65.1% (151/232), and 83.2 (193/232) at 1 week, 2 week, 1 month and 3 month, Moreover the rate of esophageal incision healing 20% (6/30), 86.7% (26/30), 96.7% (29/30) and 100%. Conclusion: Most of esophageal incisions after POEM will heal from 2 weeks to 1 months, but falling completely of the titanium clips will require a longer time.

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