scabiei isolate #20 used as inoculum The first experiment (trial

scabiei isolate #20 used as inoculum. The first experiment (trial 1) was planted

on 23rd April 2007 and the second (trial 2) on 10th August 2007. Plant establishment, tuber selection and inoculum preparation was as described for the preliminary experiment. In trial 1, there were three inoculation treatments: 10, 20 and 30 DAT, with each treatment consisting of a single spray of pathogen spore suspension. In trial 2, there were three inoculation treatments, each consisting of LY2157299 order two sprays at 5 day intervals. The first was at 3 and 8 DAT, the second 13 and 18 DAT and the third at 23 and 28 DAT. A control treatment of water only was included in both trials. Treated tubers were harvested at plant senescence and the proportion of tubers showing any disease lesions in each treatment was recorded. Each tuber was then assessed for the estimated tuber surface coverage by lesions and the depth of the deepest lesion present using the methods of Wilson et al. (1999). In the preliminary trial, occasional lesions were noted on treated tubers of ‘Desiree’, ‘Shepody’ and ‘Russet Burbank’ buy GDC-0068 following both inoculation techniques. Spore suspension sprays produced approximately twofold more lesions than the droplet inoculation method. Varieties

‘Desiree’ and ‘Shepody’ had comparable infection rate being approximately twofold greater than ‘Russet Burbank’. Subsequent experiments utilized the susceptible cultivar ‘Desiree’ with the most effective inoculation method (spore suspension spray). In both trials, individual tubers of cultivar ‘Desiree’ were successfully infected with S. scabiei

isolate #20 and typical common scab disease symptoms expressed (Fig. 2). All tubers from control treatments remained healthy. Whilst S. scabiei infection of seedling shoots and roots has been demonstrated in soil-less media (Leiner et al. 1996; Goyer et al. 1998), here we report successful infection of developing tubers in a soil-less medchemexpress media. Tuber infection rates were higher in trial 2 where double inoculations per treatment were used. The highest percentage infection and scab surface coverage was 36.6 and 3.8%, respectively, when inoculated 20 DAT in trial 1, and 66.6 and 4.6%, respectively, when inoculated at 3 and 8 DAT in trial 2 (Table 1). Inoculation of more mature tubers (trial 1 – 30 DAT, trial 2 – 23 and 28 DAT) showed a reduction in symptom expression suggesting reduced susceptibility perhaps due to increased physical resistance e.g. suberization of lenticels (Adams 1975). Whilst the mean lesion depth of the deepest lesion on infected tubers did not significantly vary between inoculation date treatments, trends suggest lesion depth increased the earlier tubers were inoculated. These results showing infection greatest during the early stage of tuber formation is in agreement with others (McIntosh 1970; Loria et al.

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