001). There were significant improvements in VO2peak after three weeks of training and supplementing across both treatment check details groups (p < 0.001; ES: SHP099 cell line 0.977). While there

were no significant difference for the improvements in VO2peak at any time point between groups, only the BA group demonstrated significant improvements from mid- to post-training and supplementing (p = 0.010) with no significant change from mid- to post- for the PL group (p = 0.118). Similar results for VO2TTE were also revealed with both groups demonstrating significant improvements from pre- to mid-testing (p < 0.001; ES: 0.983), with no difference between groups. Significant changes from mid- to post-VO2TTE were only evident in the BA group (p = 0.043). There were no significant differences among the improvements in VT between groups. Improvements from pre- to mid VT for both the PL and BA groups did not yield significance. However, the PL group was the only group to demonstrate significant improvements from mid- to post (p = 0.001). Time to exhaustion test-TWD The improvements in TWD were significant

across all time points, with no difference between groups (p > 0.05; ES: 0.898). While not significant, the delta values at both time points were greater for the BA group [pre-mid: 30.6 ± 19.9 sec; mid-post: 42.3 ± 72.1 sec] when compared to the PL group [pre-mid: 27.6 ± 22.1; mid-post: 18.6 ± 28.3]. Body Composition The physical characteristics APO866 of the subjects determined at mid-testing and after six-weeks of HIIT and supplementing are presented in Table 2. Body mass did not change significantly with supplementing or training. However, the determination of body composition with the use of air displacement plethysmography (Bod Pod®) revealed a significant improvement from pre- to mid-testing in lean body mass in only the BA group (p = 0.011; ES: 0.985) and no change in the PL group (p = 0.138). Furthermore, there were no significant changes in percent body fat (p = 0.287) or fat mass (p = 984) between treatment groups

after three and six weeks of HIIT and supplementation. Table 2 Mean ± SD values for body weight (kg), body fat (%), lean body mass (kg), and fat mass (kg) from pre-, mid-, and post-testing.   β-alanine (n = 18) Placebo Regorafenib cell line (n = 18)   Pre-testing Mid-testing Post-testing Pre-testing Mid-testing Post-testing Weight (kg) 78.8 ± 12.8 80.1 ± 13.0 79.8 ± 12.4 78.5 ± 11.3 79.3 ± 12.3 79.8 ± 11.9 Body Fat (%) 13.7 ± 6.3 13.7 ± 6.4 13.7 ± 5.6 16.1 ± 7.5 15.9 ± 8.3 16.0 ± 7.9 Lean Body Mass (kg) 67.6 ± 8.9 68.6 ± 8.6* 68.4 ± 8.4 65.5 ± 8.1 66.1 ± 8.5 65.8 ± 8.4 Fat Mass (kg) 11.3 ± 6.5 11.5 ± 6.8 11.3 ± 6.0 13.0 ± 7.1 13.1 ± 8.0 13.0 ± 7.8 *indicates a significant difference from pre- to mid-testing. (p < 0.05). Dietary Analysis There was no significant difference between groups for their supplement or training compliance rate, representing a 6.4 -3.2 g per day intake for the BA group, for the three and six weeks, respectively.