, 2010) In the present work, we explored the subset of extracell

, 2010). In the present work, we explored the subset of extracellular proteins produced by a panel of LAB and bifidobacteria frequently found in foods or that are normal inhabitants of the human GIT. We aimed to detect changes in the production of extracellular proteins as affected by the presence of cecum extract in

the culture medium. A panel of food/probiotic bacteria was used, among which representative strains for dairy starters, adjunct dairy cultures, commensal species inhabiting the human GIT, and probiotic strains were chosen. In addition, the strain B. animalis ssp. lactis R2, a strain producing a ropy exopolysaccharide that may be relevant for the food industry (Ruas-Madiedo & de los Reyes-Gavilán, 2005), was also included (Table 1) (Gasson, 1983). In our RXDX-106 nmr experimental design, different subinhibitory concentrations of cecum extract obtained from the pooled cecum contents of four healthy donors were added to the

growth culture media. The highest amount of extracellular proteins was recovered from the supernatants of bacteria cultured to stationary phase of growth. Therefore, we used GS 1101 extracellular proteins isolated in this phase for obtaining preliminary electrophoretic profiles. In general, the extracellular protein profiles of the cultures of selected bacteria were affected qualitatively by the presence and concentration of cecum extract initially added to the growth medium. Many of the new mafosfamide bands were identified as components of the cecum extract (Fig. 1, see Supporting Information, Table S1), but two of them were shown to be highly upregulated bacterial proteins: surface antigen (Imp11; accession number ZP_00121020) from Bifidobacterium longum and a small extracellular protein of unknown function (Imp23; accession number YP_193019) produced by L. acidophilus (Fig. 2a). After their identification, we could further demonstrate the induction of

the corresponding genes: the expression level of imp11 remained at a twofold higher level in the presence of cecal content along the growth curve, whereas imp23 was considerably more induced in exponential than in stationary phase (Fig. 2b). It is known that intestinal bacteria are able to react to the GIT environment by activating certain genes, normally under the control of inducible promoters (Gueimonde et al., 2009; Rivera-Amill et al., 2001; Sleator et al., 2005). Our results suggest that the expression of certain genes, whose products could be relevant for the physiology of the bacterium in the GIT, may be up- or down-regulated in conditions used in the laboratory, thus escaping analysis. In contrast, the actual relevance regarding bacteria–host interaction of proteins produced at higher amounts in nonconditioned media with respect to simulated GIT conditions should be carefully addressed. For instance, S-layer protein A from L.

SCS is consistent with Shapiro’s (1968) definition of a placebo,

SCS is consistent with Shapiro’s (1968) definition of a placebo, in that the participant does not know which treatment is being applied, and the treatment probably has no effect on the person. While there may be quibbles over specific deliveries of TMS or tCS (such as clicking from the coil, or itching at the scalp), SCS could fairly be called a placebo, especially if these factors were identical in active and sham sessions. But what about OAS? The key is the word ‘specific’: if the stimulation is delivered to a brain area that is known (inasmuch as this selleck chemicals llc is possible) not to be involved in a task, the stimulation might

indeed be considered a placebo. However, ACS differs markedly from the usual medical idea of a placebo, in that the stimulation is being delivered somewhere. While the task-related brain area may be unaffected in the OAS condition, nevertheless the person’s brain tissue is being affected in some way. While buy Opaganib the stimulation levels used in most experimental settings are well within physiologically ‘safe’ limits (Jahanshahi et al., 1997; Bikson et al., 2009; Datta et al., 2009), it is still possible that small changes in neural excitability could induce deleterious effects. There are some cases in which an active control is necessary. For example, high-intensity tACS around the frontal or occipital areas is likely to cause visual disturbances due

to stimulation of the retina or visual cortex (Kanai et al., 2008; Schutter & Hortensius, 2010). In this case the participant is always aware of the stimulated conditions. It would therefore be sensible to choose two separate electrode montages, with one over the target brain area and the other over a neutral location that would produce the same visual sensations. However, stimulating one area of the scalp is likely to feel very different from stimulating another: even a naïve participant will realize that TMS over dorsolateral prefrontal cortex has different sensory consequences

from vertex stimulation. A primary purpose of a control condition in an experiment or trial is to show the specificity of the effect to the primary condition; therefore, the control must replicate as closely as possible the ‘active’ condition but BCKDHB the hypothesized brain area should not be stimulated. In this view, OAS gives the fairest comparison of active with sham conditions, as the only difference between the conditions is the position of the electrodes or stimulating coil. We recommend that active control brain stimulation be used as a last resort, and that appropriate safety checks are employed. First, the impact of the control stimulation on the brain should be understood, ideally through current density modelling or through relating the planned stimulation parameters to known physiological measures.

It is important to obtain either tissue samples or body fluid in

It is important to obtain either tissue samples or body fluid in which the organism can be identified (category III recommendation). If induced sputum (IS) is routinely available, this can be performed initially (sensitivity 50–90%). If IS results are negative or inconclusive, then the patient should be assessed for bronchoscopy with broncho-alveolar lavage (BAL; diagnostic sensitivity >90%) [24–27]. Some may choose BAL as the first-line investigation employed. Open lung biopsy (diagnostic sensitivity 95–98%) is reserved for the occasional patient, with negative initial tests, and who is not improving

on empirical treatment [28,29]. Spontaneously expectorated sputum is not an adequate alveolar sample and should not be processed. Pneumocystis jirovecii cannot be cultured in vitro; diagnosis relies on visualization of the organism using either histochemical (typically with silver stains such selleck products as Grocott–Gomori methenamine silver stain) or immunofluorescent stains. Nucleic acid amplification techniques (NAAT) using a variety of primers have been reported with induced sputum, BAL and oral wash specimens [30–33]. In general NAAT-based tests have increased sensitivity but reduced specificity compared to visualization; and the specificity varies by protocol. In one study comparing two NAAT-based assays the sensitivities were 97–98% but specificities ranged from 68% to 96% [30]. The sensitivity is lower using samples that are obtained

from more easily obtained specimens such as sputum or oral washes. NAAT-based assays, although not widely available, can provide information on the molecular MG-132 cost epidemiology of PCP and the frequency of mutations in Pneumocystis’ dihydropteroate synthase gene (which is associated with previous exposure to sulpha- drugs – see later). Currently, no definitive Acetophenone recommendations concerning NAAT-based assays can be made. Where centres use them as part

of a diagnostic algorithm they must be interpreted with input from the testing laboratory in the light of their sensitivity and specificity. They should be combined with a definitive visualization technique (category IV recommendation). Treatment should not be delayed in a presumed case, by having to wait for a diagnostic procedure, as adequate pulmonary samples can be obtained up to 7–10 days after starting specific anti-pneumocystis therapy [34]. First-line treatment for moderate–severe PCP [PaO2≤9.3 kPa (≤70 mmHg)] is with high-dose intravenous (iv) trimethoprim-sulphamethoxazole for 21 days (co-trimoxazole, TMP-SMX) (category Ib recommendation). Co-trimoxazole is a highly effective agent when given for 21 days. It has an efficacy of at least 90% in mild disease and around 70% in more severe cases [35–38]. It has shown similar or better outcomes when compared to iv pentamidine in randomized clinical trials of treatment of PCP [35–37]. Dosing for moderate–severe PCP [PaO2≤9.3 kPa (≤70 mmHg), see Table 3.

A mutated SLCMV Rep, in which a frame shift mutation caused reten

A mutated SLCMV Rep, in which a frame shift mutation caused retention MG 132 of only 27 of the 351 amino acids, did not cause IS426 transposition in A. tumefaciens. These findings show that the multifunctional begomoviral Rep protein of SLCMV triggers transposition of IS426 in Agrobacterium. “
“Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic

distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific

primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair BMS-354825 supplier was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae selleck compound collected in its native habitat

has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field. Truffles (Tuber spp.) are ectomycorrhizal fungi producing edible hypogeous fruit bodies of economic importance. Tuber magnatum and Tuber melanosporum are some of the most prized and expensive delicacies in international haute cuisine. Tuber aestivum (including forma uncinatum) and several other Tuber spp. are less valued. Tuber aestivum (summer truffle) has been frequently overlooked in most European countries. Nowadays, it has been rediscovered in a number of habitats all over Europe (Chevalier & Frochot, 1997; Montecchi & Sarasini, 2000, Gažo et al., 2005, Pomarico et al., 2007) and is considered the most common European truffle. Soil and climatic requirements of the summer truffle can be met in many natural localities in Europe and this fungus is thus probably the easiest of all truffles to cultivate commercially. In addition, it is the only truffle species with fruit-bodies ripening advantageously from late-May up to winter (Chevalier & Frochot, 1997). These features are probably the reason for its gradually increasing commercial value. In some countries (e.g. France, burgundy truffle), T. aestivum is harvested, cultivated and marketed, whereas in others, for example, the Czech Republic or Slovakia, this species is considered critically endangered and protected by law. There, collecting of T.

A mutated SLCMV Rep, in which a frame shift mutation caused reten

A mutated SLCMV Rep, in which a frame shift mutation caused retention Natural Product Library screening of only 27 of the 351 amino acids, did not cause IS426 transposition in A. tumefaciens. These findings show that the multifunctional begomoviral Rep protein of SLCMV triggers transposition of IS426 in Agrobacterium. “
“Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic

distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific

primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair http://www.selleckchem.com/products/Trichostatin-A.html was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae Cyclin-dependent kinase 3 collected in its native habitat

has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field. Truffles (Tuber spp.) are ectomycorrhizal fungi producing edible hypogeous fruit bodies of economic importance. Tuber magnatum and Tuber melanosporum are some of the most prized and expensive delicacies in international haute cuisine. Tuber aestivum (including forma uncinatum) and several other Tuber spp. are less valued. Tuber aestivum (summer truffle) has been frequently overlooked in most European countries. Nowadays, it has been rediscovered in a number of habitats all over Europe (Chevalier & Frochot, 1997; Montecchi & Sarasini, 2000, Gažo et al., 2005, Pomarico et al., 2007) and is considered the most common European truffle. Soil and climatic requirements of the summer truffle can be met in many natural localities in Europe and this fungus is thus probably the easiest of all truffles to cultivate commercially. In addition, it is the only truffle species with fruit-bodies ripening advantageously from late-May up to winter (Chevalier & Frochot, 1997). These features are probably the reason for its gradually increasing commercial value. In some countries (e.g. France, burgundy truffle), T. aestivum is harvested, cultivated and marketed, whereas in others, for example, the Czech Republic or Slovakia, this species is considered critically endangered and protected by law. There, collecting of T.

These results suggest that modulation of PI3K signaling could con

These results suggest that modulation of PI3K signaling could contribute to improve the cognitive deficits associated with FXS. “
“In the anti-saccade task, a subject must make a saccadic eye movement in the opposite direction from a suddenly-presented visual target. This sets up a conflict between the natural tendency to make a pro-saccade towards the target and the required anti-saccade. Consequently there is a tendency to make errors, usually corrected by a second movement in the

correct anti-saccade direction. In a previous paper, we showed that a very simple model, with racing LATER (Linear Approach to Threshold at Ergodic Rate) units for the pro- and anti-directions, and a stop unit that inhibits the impending error response, could account precisely for the detailed distributions of reaction times both for correct and http://www.selleckchem.com/products/wnt-c59-c59.html error responses. However, the occurrence and timing of these final corrections have not been studied. We propose a novel mechanism: the decision race re-starts Kinase Inhibitor Library price after an error. Here we describe measurements of all the responses in an anti-saccade task, including corrections,

in a group of human volunteers, and show that the timing of the corrections themselves can be predicted by the same model with one additional assumption, that initiation of an incorrect pro-saccade also resets and initiates a corrective anti-saccade. No extra parameters are needed to predict this complex aspect of behaviour, adding weight to our proposal that we correct our mistakes by re-starting a neural decision race.

The concept of re-starting Florfenicol a decision race is potentially exciting because it implies that neural processing of one decision can influence the next, and may be a fruitful way of understanding the complex behaviour underlying sequential decisions. “
“Axonal projections in the CNS can be categorized as either crossed or uncrossed. Crossing and uncrossing of axons has been explained by attractive and repulsive molecules like Netrin-1 and Slits, which are secreted by midline structures. However, uncrossed projections can be established even in double knockout mice of slit1 and slit2 or of roundabout1 (robo1) and robo2, two receptors for Slits. Here, we found that a novel mechanism mediated by Neuropilin-2 (Nrp2) contributes to the formation of uncrossed projections of midbrain dopaminergic neurons (mDANs). Nrp2 transcriptional activities were detected in a subset of mDANs, and its protein was expressed in mDAN axons growing through the ipsilateral diencephalon. In nrp2lacZ/lacZ mice, mDAN axons aberrantly grew toward the ventral midline and even crossed it, suggesting that Nrp2 is necessary for the development of mDAN ipsilateral projections. We investigated the involvement of Semaphorin 3B (Sema3B) and Sema3F, two ligands of Nrp2, by analysing mDAN axon trajectories in single or double knockout mice.

Data on age, sex, previous test experience, HIV status, clinical

Data on age, sex, previous test experience, HIV status, clinical stage and CD4 cell count were routinely collected in all individuals testing at the mobile service. Linkage to care was assessed by telephonic or face-to-face interviews in recruited testers with CD4 counts ≤200 and 201–350 cells/μL at 4 and 12 weeks post-diagnosis, respectively. Linkage to care was defined as having attended a healthcare facility for HIV-related care. For the purpose of this analysis, individuals who tested at the mobile HCT services as part of the randomized population sero-survey and who were therefore

personally invited to test and received a voucher were defined as ‘recruited testers’. Individuals who accessed the same mobile testing unit before the survey on their own initiative were defined as ‘voluntary C646 testers’. click here All analyses were carried out using stata version 11.0 (Stata Corp. LP, College Station, TX, USA). Characteristics of recruited and voluntary

testers were compared using cross-tabulation and the χ2 test. A total of 2066 individuals attended the mobile HCT service, including 1144 (88%) of the 1300 randomly selected actively recruited survey participants and 922 voluntary testers. A total of 208 recruited and 45 voluntary testers were excluded from the analysis: 66 tested anonymously and 187 were known to be HIV positive. Therefore, 936 recruited and 877 voluntary testers were eligible for inclusion in the analysis. The mobile HCT service visited the study community on 27 days, seeing a median of 35 clients [interquartile range (IQR) 25–42] per day, prior to the survey. The same unit conducted the sero-survey over a 40-day period, seeing a median of 47 clients (IQR 38.5–55) each day. Age, sex, body mass index and prevalence of tuberculosis symptoms were not significantly different between recruited and voluntary testers (Table 1).

Significantly more voluntary testers had been tested before (72.3%) compared with recruited testers (66.9%). The proportion of individuals who had had an HIV test within the last 12 months was higher among voluntary testers (45.6%) compared with recruited testers (35.8%). The Cell press yield of cases of newly diagnosed HIV infection was significantly higher among recruited testers [10.9%; 95% confidence interval (CI) 9.0–13.1%] compared with voluntary testers (5.0%; 95% CI 3.7–6.7%) (Table 1). CD4 count distributions were different, with a larger proportion of individuals with advanced immune suppression (CD4 count ≤200 cell/μL) among recruited testers (17.8%) compared with voluntary testers (4.6%). The median CD4 count was 385 cells/μL (IQR 267–602 cells/μL) among the recruited testers and 414.5 cells/μL (IQR 309–680 cells/μL) among voluntary testers. Linkage to care was assessed in 33 (80.5%) out of 41 recruited testers with a CD4 count ≤350 cells/μL.

Although most studies have focused on serotonin 5-HT1 receptor st

Although most studies have focused on serotonin 5-HT1 receptor stimulation as an antidyskinetic strategy, targeting the serotonin transporter modulation of dopamine activity has been overlooked. Therefore, in the current study, selective serotonin reuptake inhibitors were tested for their ability to reduce l-DOPA- and apomorphine-induced dyskinesia. In Experiments 1 and 2, hemi-parkinsonian rats were primed with l-DOPA until stable dyskinesia developed. Rats

in Experiment 1 were administered the selective serotonin reuptake inhibitors paroxetine, citalopram or fluoxetine, followed by l-DOPA. Abnormal involuntary movements and forepaw adjusting steps were recorded to determine the effects of these compounds on dyskinesia and motor performance, respectively. Brains were collected on the final test day,

after which striatal and raphe monoamines were examined via high-performance MK-8669 in vivo liquid chromatography. In Experiment 2, dyskinesias were measured after selective serotonin reuptake inhibitors and apomorphine. Serotonin reuptake inhibitors dose-dependently attenuated l-DOPA- but not apomorphine-induced dyskinesia, and preserved l-DOPA efficacy. Neurochemically, serotonin transporter inhibition enhanced striatal and raphe serotonin levels and reduced its turnover, indicating a potential mechanism of action. The present learn more results support targeting serotonin transporters to improve Parkinson’s disease treatment and provide further evidence for the role of the serotonin system in l-DOPA’s effects. “
“Numerous studies have investigated the effects of lesions of the primary visual cortex (V1) on visual responses in neurons of the superficial layer of the superior colliculus (sSC),

which receives visual information from both the retina and V1. However, little is known about the changes in the local circuit dynamics of the sSC after receiving V1 lesions. Here, we show that surround inhibition of sSC neurons is transiently enhanced following V1 lesions in mice and that this enhancement may be attributed to alterations in the balance between excitatory and inhibitory inputs to sSC neurons. Extracellular recordings in vivo revealed that sSC neuronal responses to large visual stimuli were transiently Etofibrate reduced at about 1 week after visual cortical lesions compared with normal mice and that this reduction was partially recovered at about 1 month after the lesions. By using whole-cell patch-clamp recordings from sSC neurons in slice preparations obtained from mice that had received visual cortical lesions at 1 week prior to the recordings, we found cell type-dependent changes in the balance between excitation and inhibition. In non-GABAergic cells, inhibition predominated over excitation, whereas the excitation–inhibition balance did not change in GABAergic neurons.

Although most studies have focused on serotonin 5-HT1 receptor st

Although most studies have focused on serotonin 5-HT1 receptor stimulation as an antidyskinetic strategy, targeting the serotonin transporter modulation of dopamine activity has been overlooked. Therefore, in the current study, selective serotonin reuptake inhibitors were tested for their ability to reduce l-DOPA- and apomorphine-induced dyskinesia. In Experiments 1 and 2, hemi-parkinsonian rats were primed with l-DOPA until stable dyskinesia developed. Rats

in Experiment 1 were administered the selective serotonin reuptake inhibitors paroxetine, citalopram or fluoxetine, followed by l-DOPA. Abnormal involuntary movements and forepaw adjusting steps were recorded to determine the effects of these compounds on dyskinesia and motor performance, respectively. Brains were collected on the final test day,

after which striatal and raphe monoamines were examined via high-performance PD-0332991 research buy liquid chromatography. In Experiment 2, dyskinesias were measured after selective serotonin reuptake inhibitors and apomorphine. Serotonin reuptake inhibitors dose-dependently attenuated l-DOPA- but not apomorphine-induced dyskinesia, and preserved l-DOPA efficacy. Neurochemically, serotonin transporter inhibition enhanced striatal and raphe serotonin levels and reduced its turnover, indicating a potential mechanism of action. The present Inhibitor Library results support targeting serotonin transporters to improve Parkinson’s disease treatment and provide further evidence for the role of the serotonin system in l-DOPA’s effects. “
“Numerous studies have investigated the effects of lesions of the primary visual cortex (V1) on visual responses in neurons of the superficial layer of the superior colliculus (sSC),

which receives visual information from both the retina and V1. However, little is known about the changes in the local circuit dynamics of the sSC after receiving V1 lesions. Here, we show that surround inhibition of sSC neurons is transiently enhanced following V1 lesions in mice and that this enhancement may be attributed to alterations in the balance between excitatory and inhibitory inputs to sSC neurons. Extracellular recordings in vivo revealed that sSC neuronal responses to large visual stimuli were transiently P-type ATPase reduced at about 1 week after visual cortical lesions compared with normal mice and that this reduction was partially recovered at about 1 month after the lesions. By using whole-cell patch-clamp recordings from sSC neurons in slice preparations obtained from mice that had received visual cortical lesions at 1 week prior to the recordings, we found cell type-dependent changes in the balance between excitation and inhibition. In non-GABAergic cells, inhibition predominated over excitation, whereas the excitation–inhibition balance did not change in GABAergic neurons.

The standard treatment for uncomplicated UTIs is empiric therapy

The standard treatment for uncomplicated UTIs is empiric therapy with antibiotics (Hummers-Pradier & Kochen, 2000); however, the rising clinical failure rates caused by uropathogen resistance to antibiotics (Gupta, 2002; Prais et al., 2003; Foxman, 2010) has heightened the interest in the development of alternative therapies for the prevention and treatment of UTIs. A wide range of natural products have been screened for their

nutraceutical characteristics, including pomegranate (Punica granatum) (Macnab, 1992; Lansky & Newman, 2007). For example, it has been shown that pomegranate rind extract (PGRE), in combination with a range of metal salts, exhibited antimicrobial activity against Staphylococcus aureus CDK assay (Braga et al., 2005; Gould et al., 2009), E. coli, Pseudomonas aeruginosa (Duman et al., 2009), and Proteus mirabilis (McCarrell et al., 2008). We previously showed that the swimming and swarming motility of UPEC were hindered in the presence of cranberry compounds and that

this motility inhibition resulted from a decrease in the expression of fliC (Hidalgo et al., 2011). The overall aim of this study was to explore the effect of pomegranate materials (PMs) on UPEC. Three types of PMs were tested: PGRE, pomegranate tannins (PG), and pomegranate fruit powder (PGP). Specifically, we investigated whether exposure to PMs would result in the downregulation of the fliC gene of E. coli Selleck BI 6727 CFT073 and the corresponding drop in flagellin protein production and whether this would result in impaired bacterial motility. Because flagellar motility

has been suggested to enable UPEC to disseminate to the upper urinary tract (Lane et al., 2007a, b) and considering the current rising rates of antibiotic resistance, research on the effects of PMs on the gene regulation and phenotype of this ubiquitous uropathogenic bacterium is of great relevance. Escherichia coli strain CFT073 (ATCC 700928) (wild-type pyelonephritis isolate, laboratory collection) and CFT073 PfliC-lux (Lane et al., 2007a, b) (flagellin-transcription reporter vector; SB-3CT ampr) were used as the test bacteria in this study. Escherichia coli CFT073 ΔfliC (CFT073 fliC::aphA; kanr) was used as a negative control. Cultures were grown in LB medium (10 g L−1 tryptone, 10 g L−1 NaCl, and 5 g L−1 yeast extract). Planktonic bacterial cultures were incubated at 37 °C and rotary shaking at 200 r.p.m. unless otherwise indicated. Ampicillin and kanamycin were supplemented as needed at final concentrations of 100 and 50 μg mL−1, respectively. HPLC grade PG (Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island) and PGP (Navitas Naturals) were solubilized in distilled, deionized water to concentrations of 1.5 and 100 mg mL−1, respectively, and sterilized by filtration. PGRE was prepared as described by McCarrell et al. (2008).