We conclude that particular caution must be exercised when using

We conclude that particular caution must be exercised when using very high doses of LCM in patients

with significant cardial and renal risk factors. (C) 2011 Elsevier Inc. All rights reserved.”
“Fe(73.5)Si(13.5)Cu(1)Nb(3)B(9) and Fe(77.2)Si(11.2)Cu(0.8)Nb(3.3)B(7.5) buy CP-868596 nanocomposite materials consisting of nanocrystalline phase in an amorphous matrix were obtained by heat-treatment of their precursor amorphous ribbons. The influence of structural modifications induced during the heat-treatment on soft magnetic properties and magnetoimpedance (MI) effect have been studied. The structural investigations on both these ribbons revealed the presence of two phases, fine grained Fe(3)Si phase and a residual amorphous phase on heat-treatment. The maximum MI ratio obtained in the present study is 95% at f= 4 MHz, for the optimized heat-treated Fe(77.2)Si(11.2)Cu(0.8)Nb(3.3)B(7.5) ribbon. This is ascribed to the increase in magnetic permeability and decrease in coercive force and intrinsic resistivity. Moreover, a maximum magnetic field sensitivity (xi) of 8.3%/Oe

at f = 2.5MHz is obtained, for the optimized nanocrystalline Fe(73.5)Si(13.5)Cu(1)Nb(3)B(9) ribbon. This suggests that tailoring of the nanocrystalline microstructures induced by optimum heat-treatment conditions can result in obtaining excellent combinations of the magnetic permeability and resistivity. Our results indicate that these Fe-based nanocrystalline materials can be ideally buy AZD4547 used for low magnetic field and high frequency sensor applications. (C) 2011 American Institute of Physics. [doi:10.1063/1.3656450]“
“The objective of this experiment was to determine the effect of lactoferrin (Lf) supplementation of colostrum replacer (CR) fed as 1 or 2 doses on apparent efficiency of IgG absorption, serum IgG concentrations, and xylose absorption/intestinal development in neonatal Holstein bull calves. Eighty bull calves were assigned to a 2 x 4 factorial arrangement of treatments in a randomized complete block design. Calves used were from the University

of New Hampshire (n = 48) or a commercial dairy (n = 32). All calves were fed CR according to manufacturer’s recommendations +/- Lf treatment within 90 min of birth. Lactoferrin treatments were 0 (control), 0.5, 1, and 2 g/d of supplemental Lf. At 12 h of age, calves were fed a second feeding this website of CR +/- supplemental Lf or 2 L of milk replacer +/- supplemental Lf. Calves continued to be fed milk replacer +/- supplemental Lf every 12 h for the duration of study. Blood samples were collected for determination of IgG and total serum protein at 0, 6, 12, 18, 24, and 48 h. Calves, except those fed 1 dose of CR plus 1 or 2 g of Lf, had serum IgG concentrations >= 10 g/L at 24 h. Lactoferrin supplementation had no effect on serum IgG or total serum protein concentrations. Calves fed 2 doses of CR had greater serum IgG concentrations compared with calves fed 1 dose of CR.

A total

of 346 infertility patients with oligoamenorrhoea

A total

of 346 infertility patients with oligoamenorrhoea undergoing frozen-thawed embryo transfer after priming with oestradiol and vaginal progesterone gel were included. The vaginal progesterone dose was changed from 90 mg (Crinone) once a day to twice a day and the reproductive outcome during the two periods was compared. The pregnancy rate Selleckchem Entinostat increased significantly after doubling of the progesterone dose (26.7% (90 mg) versus 38.4% (180 mg); P = 0.021). Moreover, the early pregnancy loss rate decreased significantly (67.4% versus 43.7%, respectively; P = 0.014), which significantly increased the delivery rate (8.7% versus 20.5%, respectively; P = 0.002). Doubling of the vaginal progesterone gel supplementation during frozen-thawed embryo transfer cycles decreased the early pregnancy loss rate, resulting in a significantly higher delivery rate. RBMOnline (C) buy ARS-1620 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.”
“The association between some Tumor necrosis factor-alpha (TNF-alpha) promoter polymorphisms and Type 2 diabetes mellitus (T2DM) remains controversial. Ethnic differences may play a role in these conflicting results. The aim of this study was to investigate the association between -308G/A and -238G/A polymorphisms located in the

promoter region of the TNF-alpha gene and T2DM in Mexican mestizo patients. Nine hundred four individuals (259 patients with T2DM and 645 controls) were genotyped for the -308G/A and -238G/A

polymorphisms by PCR-RFLP. We found that the -238A allele increased the risk of developing T2DM in Mexican patients (OR = 1.57, 95% CI: 1.07-2.29; p = 0.018). Moreover, we found that the frequency of the GA haplotype Selleck PF-562271 (created by the -308G and -238A alleles) was significantly increased in patients with T2DM when compared with controls (OR = 1.56, 95% CI: 1.05-2.31; p = 0.026). Our results suggest that the -238G/A polymorphism and a specific haplotype (GA) are genetic risk factors for the development of T2DM in Mexican population.”
“Since 2000, a three-phase program with a final goal to complete a 1 GHz high-resolution low-/high-temperature superconductor (LTS/HTS) nuclear magnetic resonance (NMR) magnet has been conducted at the Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology (MIT). In a LTS/HTS magnet assembly, a HTS insert is placed in the cold bore of a LTS background magnet. To date, two LTS/HTS magnets have been designed, constructed, and tested: a 350 MHz (LH350) in phase 1 and a 700 MHz (LH700) in phase 2. The program’s target has recently been upgraded from the original goal of 1 GHz to a new goal of 1.3 GHz. In this paper, we present extensive performance analyses of the two LTS/HTS NMR magnets.

While the dynamic ILM approach is suitable for lifetimes exceedin

While the dynamic ILM approach is suitable for lifetimes exceeding 10 mu s, a combination with steady-state ILM is required to measure lifetime values in the range of 1 mu s. The injection dependence does not hamper a correct determination of the carrier lifetime by the dynamic evaluation procedure.”
“Polyesters (PEs) containing two heteroatoms (Si and/or Ge) in the main chain, derived

from the acid dichlorides bis(4-chloroformylphenyl) ethyl methylsilane, bis(4-chloroformylphenyl) diethylsilane, bis(4-chloroformylphenyl) diethylgermane, and bis(4-chloroformylphenyl) di-”"butylgermane and from the diphenols bis(4-hydroxyphenyl) ethylmethylsilane, bis(4-hydroxyphenyl) diethylsilane, URMC-099 manufacturer bis(4-hydroxyphenyl) diethylgermane, and bis (4-hydroxyphenyl) di-butylgermane, were synthesized under phase-transfer Alvocidib clinical trial conditions

with three quaternary ammonium salts as phase-transfer catalysts and three NaOH concentrations in the aqueous phase. PEs were characterized with IR and NMR spectroscopy, including (29)Si-NMR. In general, the yields and intrinsic viscosities were low, and in some cases, an increase in these parameters was shown as a result of the catalyst effect. An increase in the NaOH concentration caused a decrease in the yields because of a hydrolytic process. PEs with Si were thermally more stable than those with Ge. The glass-transition temperatures decreased when the side chains bonded to the heteroatoms were longer. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 114:1080-1085, 2009″
“We prepared Fe3O4 Pevonedistat inhibitor magnetic nanoparticles having diameters of approximately 12 nm by chemical coprecipitation, which were coated with three different fatty acid surfactants: oleic acid, lauric acid, and myristic acid. From x-ray diffraction, transmission electron microscopy, and Moumlssbauer spectroscopy measurements we confirmed that Fe3O4 is the only phase present in the samples. The zero field cooled magnetization curves for the nanoparticles exhibit broad

peaks, consistent with superparamagnetic blocking for the polydisperse samples, and a saturation magnetization smaller than that for bulk Fe3O4. Although there are minimal differences in the magnetic properties of the nanoparticles having different surfactants, we find significant changes in the hydrodynamic response depending on chain length. Hyperthermia measurements show considerably larger response for oleic acid-coated samples, while magneto-optical studies indicate that these samples have slower dynamics of aggregation under the influence of a dc field. These results suggest that the magnetohydrodynamic response of ferrofluids can be controlled by judiciously selecting appropriate surfactants.

This behaviour is associated with

the specific diffusion

This behaviour is associated with

the specific diffusion mechanisms of Ag and Co: while Ag diffuses preferentially via substitutional sites, diffusion of Co is dominated by interstitial jumps. The existence of so-called non-equilibrium interfaces in UFG Ti, providing an increased density of traps for the interstitially diffusing Co and simultaneously enhancing substitutionally diffusion of Ag, is confirmed by the diffusion measurements. (C) 2011 selleck American Institute of Physics. [doi:10.1063/1.3650230]“
“Serologic evidence suggests that West Nile virus (WNV) is widely distributed in horses in southern Africa. However, because few neurologic cases have been reported, endemic lineage 2 strains were postulated to be nonpathogenic in horses. Recent evidence suggests that highly neuroinvasive lineage 2 strains exist in humans and mice. To determine whether neurologic cases are being missed in southern Africa, we tested 80 serum or brain specimens from horses with unexplained fever (n = 48) and/or neurologic signs (n = 32) for WNV. From March 2007 through June 2008, using reverse transcription-PCR

(RT-PCR) and immunoglobulin (lg) M ELISA, we found WNV RNA or IgM in 7/32 horses with acute neurologic disease; 5 horses died or were euthanized. In 5/7 horses, no other pathogen was detected. DNA sequencing for all 5 RT-PCR-positive cases showed the virus belonged to Linsitinib clinical trial lineage 2. WNV lineage 2 may cause neurologic disease in horses in southern Africa.”
“Extracts containing

mature Rhizopus oryzae lipase overexpressed in Pichia pastoris (rROL) and commercial ones from the native microorganism (nROL) have been characterized. The specific activity of rROL extract was more than 40-fold higher than nROL. The presence of multiple bands of lipases around 34 kDa was detected by western blot and zymogram analysis in both extracts. Nevertheless, rROL showed a slightly lower molecular weight than nROL. The presence of hydrolytic activity not recognised as derived from a lipase was also detected in nROL at higher molecular weights.

The influence of the ionic strength on lipase activity was assayed and there was an effect both on pH and optimal temperature. Some differences OSI-906 mouse were found between the two extracts. The specificity against triacylglycerol esters and p-nitrophenol substrates was also analyzed. Similar behaviour was noted towards triacylglycerol esters; however rROL showed opposite behaviour compared to nROL towards p-nitrophenol esters with preferences for long chain derivatives.

The properties of rROL were partially different from those reported for nROL showing the influence of the pre-pro-sequence of ROL, the post-translational modifications of Pichia pastoris and the effect of an esterase in nROL powder. (C) 2011 Elsevier B.V. All rights reserved.”
“The objective of this study was to investigate the effects of various solvents on the crystallinity and thermal expansion stability of PLA film.

In the 8-channel group, it was 60 % with 12 of the 20 postoperati

In the 8-channel group, it was 60 % with 12 of the 20 postoperative motor deficit muscles being monitored. The 16-channel group

had 100 % coverage rate of postoperative motor deficit muscles.

We suggest that multi-channel monitoring of at least eight channels is desirable for intraoperative spinal cord monitoring.”
“The objective of the present research was to develop acyclovir ophthalmic emulgels using different polymers such as Pluronic F 127 (PF 127), Gelrite, Carbopol 934P, hydroxypropylmethylcellulose (HPMC K100M) and sodium carboxymethylcellulose (Na CMC). The prepared emulgel formulations were Pinometostat cell line subjected for in vitro characterization. All the prepared emulgels showed acceptable physical

properties concerning color, homogeneity, consistency, pH value and osmolality. Water absorption studies of formulations PP2 order containing pluronic F 127 showed lesser water absorption in comparison to other formulations. All the formulations showed shear thinning pseudo plastic behavior. The formulation containing pluronic F 127 of 22 % showed higher viscosity. No significant release difference was observed between in vitro release and ex vivo release using goat cornea. The rate of drug release from all the emulgels was found to be non fickian (anomalous) release. There was no macroscopic evidence of microbial growth in the media; hence it passes the test for sterility. Ocular irritation studies done on rabbits indicated that all formulations were non irritant and did not cause inflammation. Stability studies showed that the prepared emulgels remained unchanged upon storage for 3 months at refrigerated Duvelisib purchase temperature

(4-8 degrees C).”
“This review aims to determine the spectrum of cardiac findings in our fetal and pediatric Postmortem population and provide an analysis of associated extracardiac malformations and genetic abnormalities. Pediatric autopsy reports from 2003 to 2007 inclusive were reviewed and cases with cardiac pathology selected for analysis. Over the 5-year period. 119 cases (10.8%) with abnormal cardiac findings were identified from a total of 1102 postmortem examinations. Of these cardiac cases, 42% were after termination of pregnancy for fetal anomaly, 29% after fetal demise, 14% after neonatal unit death, 3% after hospital inpatient death, and 11% after sudden unexpected death. Structural abnormality cases numbered 107 (90%), with ventricular septal defect as the most common individual defect. Nonstructural abnormality cases, such as myocarditis numbered 12 (10%). Extracardiac malformations were identified in 78%. Chromosome or gene aberrations were detected in 37%.

(C) 2011 Phytochemical Society of Europe Published by Elsevier B

(C) 2011 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.”
“OBJECTIVE: To investigate the effect of obstructive sleep apnea and continuous positive airway pressure treatment on serum butyrylcholinesterase activity and ischemia-modified albumin levels.

METHODS: Thirty-two patients with obstructive sleep apnea 5-Fluoracil in vitro and 30 age-and sex-matched controls were enrolled and underwent a diagnostic polysomnogram.

The serum butyrylcholinesterase activity, ischemia-modified albumin levels, metabolic parameters, and polysomnography scores were detected and evaluated. Nine patients were studied before and after treatment with continuous positive airway pressure.

RESULTS: The serum ischemia-modified albumin levels were significantly higher and the butyrylcholinesterase activity was significantly lower in patients with obstructive sleep apnea than in the controls (p<0.001). The continuous positive airway pressure treatment decreased the modified albumin levels and elevated the buthrylcholinesterase

activity (p = 0.019 and p = 0.023, respectively). The modified albumin levels were positively correlated with the apnea-hypopnea index (r = 0.462, p = 0.008) at baseline. Elevated ischemia-modified albumin levels can be more accurate than butyrylcholinesterase activity at reflecting the presence of obstructive sleep apnea. Receiver operating characteristic curves revealed a significant difference between the areas under the curve 0.916 for ischemia-modified albumin and 0.777 for butyrylcholinesterase (z = 2.154, Adriamycin p = 0.031).

CONCLUSION: The elevated ischemia-modified albumin level was significantly associated with obstructive sleep apnea and was more sensitive than butyrylcholinesterase activity in reflecting obstructive sleep apnea. The continuous positive airway pressure treatment helped to ameliorate the imbalance.”
“Background: We investigated the relationship between C-reactive protein (CRP) and

homocysteine on follow-up and subsequent mortality in young ischemic stroke patients in a population-based Selleck Panobinostat study. Methods: Young ischemic stroke patients were followed-up on average 6 years after the index stroke. CRP and homocysteine levels were measured and risk factors were recorded, including myocardial infarction, diabetes mellitus, hypertension, smoking, alcoholism, and cancer. Stroke outcome was measured using the modified Rankin Scale score. Subsequent survival was obtained by examining the official population registry. Cox regression analyses were performed. Results: In total, 198 patients were included in this study (82 [41%] women and 116 [59%] men). The mean age on follow-up was 47.8 years. In total, 36 (18.2%) patients died during the subsequent mean follow-up of 12.4 years. Cox regression analysis revealed that mortality was associated with CRP (hazard ratio [HR] 1.05; P = .001) and homocysteine levels (HR 1.

While extrinsic AD is the classical type with high prevalence, th

While extrinsic AD is the classical type with high prevalence, the incidence of intrinsic AD is approximately 20% with female predominance. The clinical features of intrinsic AD include relative late onset, milder severity, and Dennie-Morgan folds, but

no ichthyosis vulgris or palmar hyperlinearity. The skin barrier is perturbed in the extrinsic, but not intrinsic type. Filaggrin gene mutations are not a feature of intrinsic AD. The intrinsic type is immunologically characterized by the lower expression of interleukin (IL)-4, IL-5, and IL-13, and the higher expression of interferon-gamma. It is suggested that intrinsic AD patients are not sensitized with protein allergens, which induce Th2 responses, but with other antigens, and metals might be one of the candidates www.selleckchem.com/products/AZD8931.html of such antigens. (C) 2010 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.”
“A significant number of women with migraine has to face the choice of reliable hormonal contraception during their fertile life. Combined hormonal contraceptives

(CHCs) may be used in the majority of women with headache and migraine. However, they carry a small, but significant vascular risk, especially in migraine with aura (MA) and, eventually in migraine Fosbretabulin without aura (MO) with additional risk factors for stroke (smoking, hypertension, diabetes, hyperlipidemia and thrombophilia, age over 35 years). Guidelines recommend progestogen-only contraception as an alternative safer option because it does not seem to be associated with an increased risk of venous thromboembolism (VTE) and ischemic stroke.

Potentially, the maintenance of stable estrogen level by the administration of progestins in ovulation inhibiting dosages may have a positive influence of nociceptive threshold in women with migraine. Preliminary

evidences based on headache diaries in migraineurs suggest that the progestin-only pill containing desogestrel Compound C inhibitor 75 mu g has a positive effect on the course of both MA and MO in the majority of women, reducing the number of days with migraine, the number of analgesics and the intensity of associated symptoms. Further prospective trials have to be performed to confirm that progestogen-only contraception may be a better option for the management of both migraine and birth control. Differences between MA and MO should also be taken into account in further studies.”
“In expansion cooling phase of pinched nitrogen plasma generated by fast capillary discharge, it might be possible to realize lasing a Blamer alpha recombination SXRL, which requires a rapid cooling of nonequilibrium plasma.

Free recall of the word list and other psychological and physical

Free recall of the word list and other psychological and physical responses were assessed on day 3.

Results: Oral administration of propranolol before reactivation of the word list impaired reconsolidation of drug-related positive and negative but not neutral words in abstinent heroin addicts, and these impairments critically depended on reactivation of the word list.


This study extends earlier reports that a p-adrenergic receptor antagonist affects the drug-related memory reconsolidation process. learn more Our findings may have important implications for the understanding and treatment of persistent and abnormal drug-related memories in abstinent heroin addicts. (C) 2011 Elsevier Ireland Ltd. All rights reserved.”
“Background: Obese individuals may be at increased risk of iron deficiency (ID), but it is unclear whether this is due to poor dietary iron intakes or to adiposity-related inflammation.

Objective: The aim of this study was to examine the relations between body

mass index (BMI), dietary iron, PF-3084014 and dietary factors affecting iron bioavailability, iron status, and inflammation [C-reactive protein (CRP)] in a transition country where obesity and ID are common.

Design: Data from the 1999 Mexican Nutrition Survey, which included 1174 children (aged 5-12 y) and 621 nonpregnant women (aged 18-50 y), were analyzed.

Results: The prevalence of obesity was 25.3% in women and 3.5% in children. The prevalence of ID was significantly (P < 0.05) higher in obese women and children compared with normal-weight subjects [odds ratios (95% CIs): 1.92 (1.23, 3.01) and 3.96 (1.34, 11.67) for women and children, respectively]. Despite similar dietary iron intakes in the 2 groups, serum iron concentrations were lower in obese women than Small molecule library molecular weight in normal-weight women (62.6 +/- 29.5 compared with 72.4 +/- 34.6 mu g/dL; P = 0.014), and total-iron-binding capacity was higher in obese

children than in normal-weight children (399 +/- 51 compared with 360 +/- 48 mu g/dL; P < 0.001). CRP concentrations in obese women and children were 4 times those of their normal-weight counterparts (P < 0.05). CRP but not iron intake was a strong negative predictor of iron status, independently of BMI (P < 0.05).

Conclusions: The risk of ID in obese Mexican women and children was 2-4 times that of normal-weight individuals at similar dietary iron intakes. This increased risk of ID may be due to the effects of obesity-related inflammation on dietary iron absorption. Thus, ID control efforts in Mexico may be hampered by increasing rates of adiposity in women and children. Am J Clin Nutr 2011;93:97583.”
“We report a case series of 11 patients with severe E. faecium infections treated with daptomycin. All strains were resistant to ampicillin (MIC >8 mg/l), but susceptible to vancomycin. Seven out of 11 strains were also highly resistant to gentamicin (MIC >500 mg/l).

4, 93 5, 91 2 and 90 3% respectively The limit of quantification

4, 93.5, 91.2 and 90.3% respectively. The limit of quantification was 25 ng/mL for I and IV, 10 ng/mL for II and III and 100 ng/mL for V respectively. The within

day and day-to-day precision for all the compounds were < 10%. The validated HPLC method herein was applied for pharmacokinetic studies and the main pharmacokinetic parameters were: t(1/2) (hr) 5.8, 4.3, 2.9, 5.7 and 7.3, C-max (ng/mL) 594.9, 1542.9 1659.9, 208.9 and 3040.4; T-max (hr) 4.7, 1.0, 1.0, 3.5 and 2.3; AUC(0-infinity). (ng hr/mL) 5040, 5893, 9260, 1064 and 27233 for I, II, III, IV and V respectively. The developed method was suitable for pharmacokinetic studies and this preliminary study also revealed significant absorption after oral dosing in rats.”
“The possible connection between chronic oral inflammatory processes, such as apical periodontitis and periodontal disease (PD), and AL3818 ic50 systemic health is one of the most interesting aspects faced by the medical and dental scientific community. Chronic apical periodontitis shares important characteristics with PD: 1) both are chronic infections of the oral cavity, 2) the Gram-negative anaerobic microbiota found in both diseases is comparable,

and 3) in both infectious processes increased local levels of inflammatory mediators may have an impact LY2157299 cell line on systemic levels. One of the systemic disorders linked to PD is diabetes mellitus (DM); is therefore plausible to assume that chronic apical periodontitis and endodontic treatment are also associated with DM. The status of knowledge regarding the relationship between DM and endodontics is reviewed. Upon review, we conclude that there are data in the literature that associate DM with a higher prevalence of periapical lesions, greater size of the osteolityc lesions, greater likelihood of asymptomatic learn more infections and worse prognosis for root filled teeth. The results of some studies suggest that periapical disease may contribute to diabetic metabolic dyscontrol.”
“Recent research in cerebellar cognitive and linguistic functions makes plausible the idea that the cerebellum is involved in processing temporally contiguous linguistic

input. In order to assess this hypothesis, a simple lexical decision task was constructed to study whether the effect of transcranial magnetic stimulation on two different cerebellar sites would have a selective impact on associative as opposed to semantic priming. This is the first experiment applying transcranial magnetic stimulation of the cerebellum to a linguistic task. The results show a selective drop in lexical decision accuracy after stimulation of a medial cerebellar site in the first session of participation. Most importantly, they also demonstrate a selective increase of associative priming sizes after stimulation of the same site that cannot be attributed to changes in sensorimotor performance or in accuracy rates. The finding is discussed within the context of domain-general associative cerebellar computations.

In this case, HPMCAS can form H bonds with griseofulvin directly;

In this case, HPMCAS can form H bonds with griseofulvin directly; the addition of PHPMA click here to the solid dispersion may enhance the stability of the amorphous griseofulvin due to greater interaction with griseofulvin. The X-ray powder diffraction results showed that griseofulvin (binary and ternary solid dispersions) remained amorphous for more than 19 months stored at 85% RH compared with the spray-dried griseofulvin which crystallized totally within 24 h at ambient conditions. The Fourier transform infrared scan showed that

griseofulvin carbonyl group formed hydrogen bonds with the hydroxyl group in the HPMCAS, which could explain the extended stability of the drug. Further broadening in the peak could be seen when PHPMA was added to the solid dispersion, which indicates stronger interaction. The glass transition

temperatures increased in the ternary solid dispersions regardless of HPMCAS grade. The dissolution rate of the drug in the solid dispersion (both binary and ternary) has significantly increased when compared with the dissolution profile of the spray-dried griseofulvin. These results reveal significant stability of the amorphous form due to the hydrogen bond formation with the polymer. The addition of the third polymer improved the stability but had a minor impact on dissolution.”
“Onion anthracnose, caused by Colletotrichum gloeosporioides, is one of the main diseases of onions in the State of Pernambuco. We examined the pathogenicity of 15 C. gloeosporioides strains and analyzed their genetic variability using RAPDs and internal

transcribed this website spacers (ITS) of the rDNA region. Ten of the strains were obtained from substrates and hosts other than onion, including chayote (Sechium edule), guava (Psidium guajava), pomegranate (Punica granatum), water from the Capibaribe River, maracock (Passiflora AC220 sp), coconut (Cocus nucifera), surinam cherry (Eugenia uniflora), and marine soil; five isolates came from onions collected from four different regions of the State of Pernambuco and one region of the State of Amazonas. Pathogenicity tests were carried out using onion leaves and bulbs. All strains were capable of causing disease in leaves, causing a variable degree of lesions on the leaves; four strains caused the most severe damage. In the onion bulb tests, only three of the above strains caused lesions. Seven primers of arbitrary sequences were used in the RAPD analysis, generating polymorphic bands that allowed the separation of the strains into three distinct groups. The amplification products generated with the primers ITS1 and ITS4 also showed polymorphism when digested with three restriction enzymes, DraI, HaeIII and MspI. Only the latter two demonstrated genetic variations among the strains. These two types of molecular markers were able to differentiate the strain from the State of Amazonas from those of the State of Pernambuco.