7,23 Although patient selection bias for IFN treatment versus no treatment had been noted in the previous studies, the results suggest the possibility that IFN therapy reduces the development of HCC in HCV patients. Several historical data in Japan suggest that IFN therapy reduces the development of HCC in HCV patients.24-26 Second, HCC occurred with statistical significance when the following characteristics were present: non-SVR, advanced age, cirrhosis, TAI of

≥200 kg, male sex, and T2DM. T2DM caused a 1.73-fold enhancement in HCC development. Sotrastaurin in vivo Several authors have reported an increased risk of HCC among patients with the following characteristics: non-SVR, cirrhosis, male sex, advanced age, and T2DM.24-28 this website Our results show that physicians in charge of aged male patients with non-SVR, advanced fibrosis, TAI of ≥200 kg, and T2DM should pay attention to the development of HCC after IFN therapy. In addition, maintaining a mean HbA1c level of <7.0% during follow-up reduced

the development of HCC. This result indicates that stringent control of T2DM is important for protecting the development of HCC. Third, the development rate of HCC per 1,000 person years was about 1.55 in 1,751 patients with chronic hepatitis at baseline and SVR. In these patients, the risk factors associated with HCC were advanced age, male sex, TAI, and T2DM. We compared the HCC development rate in patients with chronic hepatitis at baseline and SVR to the general population. A total of 5,253 individuals without HCV antibody and hepatitis B surface antigen,

who underwent annual multiphasic health screening examinations in our hospital were evaluated as controls. Individuals with either of the following criteria were excluded: (1) illness that could seriously reduce their life expectancy or (2) history of carcinogenesis. They were selected by matching 3:1 with patients who had chronic hepatitis at baseline and SVR for age, sex, T2DM, and follow-up periods. In control individuals, the mean age was 51.7 years; the prevalence (number) MCE of male patients was 61.8% (3,246); the prevalence (number) of T2DM patients was 4.2% (222); the mean follow-up period was 8.0 years. The number of development of HCC in control individuals was only five. This result suggests that the development rate of HCC in patients with chronic hepatitis at baseline and SVR is higher than that in the general population. Fourth, HCC accounted for 33.3% in SVR patients and 73.6% in non-SVR patients. According to Matsuda et al.,29 the outbreak of malignancies in the Japanese male population was observed in the following order in 2005: gastric cancer 20.4% > colon cancer 16.0% > lung cancer 15.4% > prostate cancer 10.9% > HCC 7.4%. On the other hand, the outbreak of malignancies in the Japanese female population was observed in the following order in 2005: breast cancer 18.0% > colon cancer 16.2% > gastric cancer 13.6% > lung cancer 9.3% > uterine cancer 6.8%.

Among these preparations, S-allyl cysteine (SAC), one of the major organic garlic compounds that had been known to possess a powerful anti-oxidant property, can be candidate for potential formula compared with other organosulfur compounds, including diallyl bisulfide, diallyl trisulfide (DAT3S), or diallyl-tetrasulfide (DAT4S).[9, 10] Studies in mouse and rodent models have shown that SAC can be rapidly absorbed in the GI tract and has very low acute/subacute toxicity (LD50 value > 54.7 mM/kg oral; > 20 mM/kg intraperitoneal), which is 30-fold less toxic than other typical garlic compounds, such as allicin http://www.selleckchem.com/JAK.html and diallyl disulfide.[11, 12] Moreover, SAC is two hydrophilic cysteine-containing compounds

naturally formed in Z-VAD-FMK molecular weight garlic. It has been shown to exert anti-inflammatory and anti-oxidative effects in various models, but never been tried against GI inflammation.[13] Recently, phytoceuticals or phytochemicals were proven to possess the ability to orchestrate gastric inflammation more globally through histone deacetylator (HDAC) inhibitory activity, as well as potentiate the host defensive phase enzyme induction like heme oxygenase-1 (HO-1) through nuclear factor erythroid-derived 2-related factor (Nrf2) transcriptional activation, a transcription factor that in humans

is encoded by the NFE2L2 gene, of which Nrf2 anti-oxidant response pathway is “the primary cellular defense” against the cytotoxic effects of oxidative stress, in our study provoked by indomethacin. The object of our study was either to document the preventive efficacy of MCE公司 SAC against indomethacin-induced gastric damage or to identify the underlying molecular mechanisms on how SAC could impose protection. An animal model of indomethacin-induced gastric damage model was established, and tumor necrosis

factor-α (TNF-α)-stimulated cell models were used for current experiment. All chemical reagents were obtained from Sigma (St. Louis, MO, USA). Synthetic SAC was provided by Pharmaking Co., Ltd (Seongnam, Korea). Rebamipide was provided by Otsuka Pharmaceutical Co., Ltd (Tokushima, Japan). PD98095 and SB202190 were provided from Calbiochem (Billerica, MA, USA). Western blotting detection reagents were obtained from Amersham Biotechnology (Bucks, UK). Antibodies for COX-2, β-actin, inducible nitric oxide synthase (iNOS), α-tubulin, superoxide dismutase-1 (SOD-1), glutathione peroxidase-2 (GPX-2), glutathione-S-transferase-π (GST-π), Nrf2, γ-Glutamylcysteine Synthetase (γ-GCS), NAD(P)H:quinone oxidoreductase-1 (NQO-1), phospho-extracellular-signal-regulated kinase (pERK), and ERK were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for calcium-dependent phospholipase A2 (cPLA2), phosphor-IκBα, IκBα, p65, p50, phospho-p38, and p38 were purchased from Cell Signaling Technology (Denver, MA, USA). Antibody for heme oxygenase 1 (HO-1) was from R&D Systems, Inc. (Minneapolis, MN, USA).

Among these preparations, S-allyl cysteine (SAC), one of the major organic garlic compounds that had been known to possess a powerful anti-oxidant property, can be candidate for potential formula compared with other organosulfur compounds, including diallyl bisulfide, diallyl trisulfide (DAT3S), or diallyl-tetrasulfide (DAT4S).[9, 10] Studies in mouse and rodent models have shown that SAC can be rapidly absorbed in the GI tract and has very low acute/subacute toxicity (LD50 value > 54.7 mM/kg oral; > 20 mM/kg intraperitoneal), which is 30-fold less toxic than other typical garlic compounds, such as allicin selleck kinase inhibitor and diallyl disulfide.[11, 12] Moreover, SAC is two hydrophilic cysteine-containing compounds

naturally formed in check details garlic. It has been shown to exert anti-inflammatory and anti-oxidative effects in various models, but never been tried against GI inflammation.[13] Recently, phytoceuticals or phytochemicals were proven to possess the ability to orchestrate gastric inflammation more globally through histone deacetylator (HDAC) inhibitory activity, as well as potentiate the host defensive phase enzyme induction like heme oxygenase-1 (HO-1) through nuclear factor erythroid-derived 2-related factor (Nrf2) transcriptional activation, a transcription factor that in humans

is encoded by the NFE2L2 gene, of which Nrf2 anti-oxidant response pathway is “the primary cellular defense” against the cytotoxic effects of oxidative stress, in our study provoked by indomethacin. The object of our study was either to document the preventive efficacy of MCE公司 SAC against indomethacin-induced gastric damage or to identify the underlying molecular mechanisms on how SAC could impose protection. An animal model of indomethacin-induced gastric damage model was established, and tumor necrosis

factor-α (TNF-α)-stimulated cell models were used for current experiment. All chemical reagents were obtained from Sigma (St. Louis, MO, USA). Synthetic SAC was provided by Pharmaking Co., Ltd (Seongnam, Korea). Rebamipide was provided by Otsuka Pharmaceutical Co., Ltd (Tokushima, Japan). PD98095 and SB202190 were provided from Calbiochem (Billerica, MA, USA). Western blotting detection reagents were obtained from Amersham Biotechnology (Bucks, UK). Antibodies for COX-2, β-actin, inducible nitric oxide synthase (iNOS), α-tubulin, superoxide dismutase-1 (SOD-1), glutathione peroxidase-2 (GPX-2), glutathione-S-transferase-π (GST-π), Nrf2, γ-Glutamylcysteine Synthetase (γ-GCS), NAD(P)H:quinone oxidoreductase-1 (NQO-1), phospho-extracellular-signal-regulated kinase (pERK), and ERK were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for calcium-dependent phospholipase A2 (cPLA2), phosphor-IκBα, IκBα, p65, p50, phospho-p38, and p38 were purchased from Cell Signaling Technology (Denver, MA, USA). Antibody for heme oxygenase 1 (HO-1) was from R&D Systems, Inc. (Minneapolis, MN, USA).

This might account for the observed differences in ApoR2 expression, revealing a delicate role for adiponectin in hepatic inflammation, ballooning, and apoptosis. In this context, TNF-α is known to repress adiponectin

expression and, among other mechanisms, ApoR2 activation induces phosphorylation of AMP-activated protein kinase (AMPK), increases phosphorylation of c-Jun-N-terminal-kinase (JNK), and activates peroxisome proliferator-activated receptor α (PPARα) signaling.50, 51 Our study failed to show a prognostic value for adiponectin to predict NASH, but adiponectin levels were significantly decreased in NASH and AUROC calculations revealed a modest, yet significant diagnostic value for adiponectin. Analysis of data related selleck chemical to an optimal cutoff value to determine further proved an effect of adiponectin on CD95/Fas, histological features of NASH, as well as BA

transport related genes. Several studies observed alterations in BA and adiponectin levels, yet to our knowledge, we are the first to demonstrate a potential direct effect of adiponectin and its receptor on BA homeostasis in NASH patients.52 In conclusion, our results show that serum levels of BAs are increasing in NASH and BA transport, Decitabine mouse as well as synthesis is markedly dysregulated in NAFLD. The up-regulation of the BA importer NTCP and the key enzyme in synthesis CYP7A1 in NAFLD and 上海皓元医药股份有限公司 hepatoma cells treated with FFAs indicates a dysfunctional repression of target genes by SHP. We could also show that adiponectin is inversely correlated with serum BAs and hepatocellular death and a potential effect of adiponectin on BA homeostasis-related genes, especially CYP7A1. While we provide a hint connecting BA metabolism, hepatocellular cell death, and adipocytokines, the exact mechanisms remain unknown. Further studies will aim to identify the involved pathways and distinct points of application to disrupt the vicious cycle of hepatic steatosis and its sequelae. We thank Mrs. Mechthild Beste and Claudia Gottier for technical expertise and

determination of bile acid concentrations. Additional Supporting Information may be found in the online version of this article. “
“In our previous study, the SLCO1B1 521TT genotype and the SLCO1B1*1b haplotype were significantly associated with the risk of peptic ulcer in patients taking low-dose aspirin (LDA). The aim of the present study was to investigate pharmacogenomic profile of LDA-induced peptic ulcer and ulcer bleeding. Patients taking 100 mg of enteric-coated aspirin for cardiovascular diseases and with a peptic ulcer or ulcer bleeding and patients who also participated in endoscopic surveillance were studied. Genome-wide analysis of single nucleotide polymorphisms (SNPs) was performed using the Affymetrix DME Plus Premier Pack.

Methods: Their ability to induce the production of immunoglobulin E (IgE) specific was evaluated using an assay of homologous passive cutaneous anaphylaxis. Furthermore, an allergen recognized as the lupine (Lupinus angustifolius) was included

as a control. Results: The analysis by SDS-PAGE of pigeon pea protein showed four polypeptide bands from 75 to 50 KDa and approximately to 35 KDa. Sensitization tests with both protein isolates, from green beans and dry beans of pigeon pea did not induce the production of specific IgE in the 77.8% and 75% of cases, respectively. The pigeon pea protein stimulated the production of immunoglobulin G (IgG), determined by a specific ELISA. Conclusion: The pigeon pea

protein showed a reduced capability as an allergen in this in vivo model; its use can be an alternative to lupine and soybean flour. Nevertheless, other tests in Caspase inhibitor animal models via the gastrointestinal tract and use of adjuvants are necessary. Key Word(s): 1. allergies; 2. leguminous; 3. pigeon pea; 4. proteins; Presenting Author: Md. Ariful Haque Mollik Corresponding Author: Md. Ariful Haque Mollik Affiliations: Peoples Integrated Alliance Objective: Scientists are searching for new leads from the natural sources to combat different diseases. Until recently an insignificant part of the plants has been scientifically evaluated for their medicinal values. The investigations were undertaken to discover new drugs from the natural sources. Celsia coromandelina J.König ex Rottb. belongs to the plant family Scrophulariaceae Juss. and is widely distributed PFT�� price throughout the Bangladesh. It is locally known as Kukurmota. It is locally used for the remedy of killer diseases as well as debilitating diseases. The investigations examined the anti-inflammatory, antioxidant, and antibacterial effects of its all-parts. Methods: The 75% ethanol extract was tested MCE公司 for anti-inflammatory effect using the

carrageenan-induced edema in Wistar rats. Free radical scavenging, total antioxidant, and total phenol content were assessed spectrophotometrically. The extract was tested for antibacterial activities using the agar well diffusion method and micro dilution assays. Results: The 75% ethanol extract gave a maximal inhibition of edema by 75.50% at 30 mg/kg. The total antioxidant capacities expressed in terms of ascorbic acid was 0.610 mg/g dry weight. The total phenol in terms of tannic acid was 7.50 mg/g dry weight. The extract also demonstrated free radical scavenging activities yielding half maximal inhibitory concentration (IC50) value of 1.175 mg/mL. The all-parts extract however, showed selective antibacterial activities, inhibiting growth of two microorganisms: Bacillus subtilis Ehrenberg, and Bacillus thuringiensis Berliner. The minimum inhibitory concentrations (MICs) were 500 and 1000 μg/mL respectively.

Since Taylor (1960) used Bermuda’s Meredithia crenata to illustrate K. limminghei for his concept of the species in the western Atlantic, subsequent workers may have followed his concept of the species over the past half-century. For example, a specimen from

Puerto Rico published as K. limminghei (Ballantine et al. 2011) is also attributable to Meredithia, being genetically different but most closely related to M. crenata (Saunders unpublished data). As the Bermuda specimens are now a new species in a different genus, it remains uncertain what true K. limminghei looks like at maturity and to what it is related. A sequenced collection from Guadeloupe is necessary to taxonomically understand this poorly known species and to know whether other workers’ concept of the taxon from other Caribbean sites is correct (e.g., Littler and Littler 2000). MK-8669 clinical trial In our genetic analyses, Meredithia crenata PD98059 clustered with M. microphylla, Psaromenia berggrenii (J. Agardh) D’Archino, W.A. Nelson et Zuccarello, Cirrulicarpus nanus, and several novel molecular species of Meredithia and Psaromenia from Korea, the Philippines, Lord Howe Island, Tasmania and Western Australia in a strongly supported clade (Figs. 1 and 2). Our molecular results are thus consistent with our morphological observations in assigning our Bermudian species to

the genus Meredithia. Furthermore, our molecular results corroborate the relationship of M. microphylla, M. crenata, and P. berggrenii in the partial LSU rDNA tree shown by D’Archino et al. (2011) (fig. 2, M. crenata listed as K. limminghei). Meredithia guiryorum G.W. Saunders et C.W. Schneid. sp. nov. (Fig. 6, A and B) Description: Plants in small MCE clusters of simple blades. Individuals stipitate, stipes ~0.5–1.0 mm wide and 1–2 mm tall; blades nonpeltate, irregular in outline, typically taller than wide 1.0–2.5 cm in diameter, not anastomosing(?) (Fig. 6A). Blades 200–270 μm thick in longitudinal section near the margin, composed of a moderately dense filamentous medulla with common, darkly staining stellate medullary cells observed throughout the section (Fig. 6B).

Inner cortex of two to three cell layers, outer cortex with one to two layers of slightly larger (4–5 μm wide, 5.0–7.5 μm tall) versus two to three layers of slightly smaller (2.5–5.0 μm wide, 5–6 μm tall) cells on the ventral and dorsal surfaces respectively (Fig. 6B). Reproduction not observed. Best identified by comparison with the type COI-5P barcode sequence (GenBank: KC157616). Type collection: Coll. G.W. Saunders (GWS)/K. Dixon (KD)/R. Withall (RW), November 23, 2010, North Head Gutters, Lord Howe, I., Australia, 31.52439° S, 159.04204° E, depth 15 m on rock. Holotype, UNB [GWS023256, BOLD OZSEA1125-10] (Fig. 6, A and B). Isotypes, UNB [GWS023196, GWS023198, GWS023199, GWS023220]. Additional collections (Paratypes): Listed in Table 1.

Since Taylor (1960) used Bermuda’s Meredithia crenata to illustrate K. limminghei for his concept of the species in the western Atlantic, subsequent workers may have followed his concept of the species over the past half-century. For example, a specimen from

Puerto Rico published as K. limminghei (Ballantine et al. 2011) is also attributable to Meredithia, being genetically different but most closely related to M. crenata (Saunders unpublished data). As the Bermuda specimens are now a new species in a different genus, it remains uncertain what true K. limminghei looks like at maturity and to what it is related. A sequenced collection from Guadeloupe is necessary to taxonomically understand this poorly known species and to know whether other workers’ concept of the taxon from other Caribbean sites is correct (e.g., Littler and Littler 2000). selleck screening library In our genetic analyses, Meredithia crenata Torin 1 clustered with M. microphylla, Psaromenia berggrenii (J. Agardh) D’Archino, W.A. Nelson et Zuccarello, Cirrulicarpus nanus, and several novel molecular species of Meredithia and Psaromenia from Korea, the Philippines, Lord Howe Island, Tasmania and Western Australia in a strongly supported clade (Figs. 1 and 2). Our molecular results are thus consistent with our morphological observations in assigning our Bermudian species to

the genus Meredithia. Furthermore, our molecular results corroborate the relationship of M. microphylla, M. crenata, and P. berggrenii in the partial LSU rDNA tree shown by D’Archino et al. (2011) (fig. 2, M. crenata listed as K. limminghei). Meredithia guiryorum G.W. Saunders et C.W. Schneid. sp. nov. (Fig. 6, A and B) Description: Plants in small 上海皓元医药股份有限公司 clusters of simple blades. Individuals stipitate, stipes ~0.5–1.0 mm wide and 1–2 mm tall; blades nonpeltate, irregular in outline, typically taller than wide 1.0–2.5 cm in diameter, not anastomosing(?) (Fig. 6A). Blades 200–270 μm thick in longitudinal section near the margin, composed of a moderately dense filamentous medulla with common, darkly staining stellate medullary cells observed throughout the section (Fig. 6B).

Inner cortex of two to three cell layers, outer cortex with one to two layers of slightly larger (4–5 μm wide, 5.0–7.5 μm tall) versus two to three layers of slightly smaller (2.5–5.0 μm wide, 5–6 μm tall) cells on the ventral and dorsal surfaces respectively (Fig. 6B). Reproduction not observed. Best identified by comparison with the type COI-5P barcode sequence (GenBank: KC157616). Type collection: Coll. G.W. Saunders (GWS)/K. Dixon (KD)/R. Withall (RW), November 23, 2010, North Head Gutters, Lord Howe, I., Australia, 31.52439° S, 159.04204° E, depth 15 m on rock. Holotype, UNB [GWS023256, BOLD OZSEA1125-10] (Fig. 6, A and B). Isotypes, UNB [GWS023196, GWS023198, GWS023199, GWS023220]. Additional collections (Paratypes): Listed in Table 1.

Conclusion: Serrated polyps seem to be markers of advanced colonic neoplasia and synchronous cancer. These patients should be regarded as alternate accelerated pathway of colorectal neoplasia and undergo more frequent surveillance colonoscopies then previously thought. (1)  Rondagh et al. Endoscopy 2011; 43 Key Word(s): 1. Serrated Polyps; 2. Colorectal Neoplasia; 3. Serrated Polyposis; Presenting Author: SUNLI YING Corresponding Author: SUNLI YING Affiliations: The First Affiliated Hospital of Harbin Medical University Objective: To investigate the therapeutic effect of saccharomyces boulardii (Sb) in ulcerative colitis through observation of it’s serum level of IL-8 and

IFN-γin TNBS-induced Proteases inhibitor rat colitis. Methods: forty male Wister rats (250–300 g) were randomized into four groups containing ten rats each, namely, colitis groups (group A and B and C), normal control group (group D).Then group A was given SB(800 mg/kg.d),group B was given SASP(200 mg/kg.d),while group C was given 1 ml normal saline for seven days. All of the rats were anesthetized, take blood and colon tissue. Expression of serum level IL-8 and IFN-γwere detected using ELISA, the intestinal tissue were detected by immunohistochemical staining. Results: Campared with group C, DAI scores(1.62 ± 0.73, 1.34 ± 0.605

vs 2.93 ± 0.752) and the serum level IL-8 and IFN-γ (536.32 ± 38.916, 400.38 ± 34.146 vs 783.05 ± 49.522; 328 ± 23.166, Rapamycin cost 196 ± 25.642 vs 492 ± 44.244)and the intestinal tissue level IL-8 and IFN-γwere notably lower in group A and group B(P < 0.01),

they were expressed much higher in group A campared with group D (P < 0.01). Campared group A and B, the serum level IL-8 and IFN-γand the intestinal tissue level IFN-γ were higher (P < 0.05), but the intestinal tissue level IL-8 and DAI scores were expressed no difference (P = 0.314, P = 0.139). Conclusion: There is therapeutic effect with Sb for UC, which 上海皓元 may be related to reduce serum and intestinal tissue level of IL-8 and IFN-γ. Key Word(s): 1. ulcerative colitis; 2. SASP; Presenting Author: CHANG QING YIN Corresponding Author: CHANG QING YIN Objective: To observe the expression and clinical significance of E-cadherin (E-CAD) and Matrix metalloproteinase-7 (MMP-7)in colorectal carcinoma. Methods: Expression of E-CAD and MMP-7 in 20 cases of normal colorectal mucosa, 34 cases of colorectal adenoma and 62 cases of colorectal carcinoma was determined by immunohistochemical staining, and relationship between E-CAD and MMP-7 expression and pathological features in 62 cases of colorectal carcinoma. Results: The positive expression of E-CAD in normal colorectal mucosa was significantly higher than those in adenoma and colorectal carcinoma. In colorectal carcinoma and adenoma, the positive expression of MMP-7 was significantly higher than those in normal colorectal mucosa. The expression of E-CAD was decreased and that of MMP-7 was increased with Dukes stage and depth of invasion rising and lymph node transferring.

Conclusion: Serrated polyps seem to be markers of advanced colonic neoplasia and synchronous cancer. These patients should be regarded as alternate accelerated pathway of colorectal neoplasia and undergo more frequent surveillance colonoscopies then previously thought. (1)  Rondagh et al. Endoscopy 2011; 43 Key Word(s): 1. Serrated Polyps; 2. Colorectal Neoplasia; 3. Serrated Polyposis; Presenting Author: SUNLI YING Corresponding Author: SUNLI YING Affiliations: The First Affiliated Hospital of Harbin Medical University Objective: To investigate the therapeutic effect of saccharomyces boulardii (Sb) in ulcerative colitis through observation of it’s serum level of IL-8 and

IFN-γin TNBS-induced Selleckchem EGFR inhibitor rat colitis. Methods: forty male Wister rats (250–300 g) were randomized into four groups containing ten rats each, namely, colitis groups (group A and B and C), normal control group (group D).Then group A was given SB(800 mg/kg.d),group B was given SASP(200 mg/kg.d),while group C was given 1 ml normal saline for seven days. All of the rats were anesthetized, take blood and colon tissue. Expression of serum level IL-8 and IFN-γwere detected using ELISA, the intestinal tissue were detected by immunohistochemical staining. Results: Campared with group C, DAI scores(1.62 ± 0.73, 1.34 ± 0.605

vs 2.93 ± 0.752) and the serum level IL-8 and IFN-γ (536.32 ± 38.916, 400.38 ± 34.146 vs 783.05 ± 49.522; 328 ± 23.166, SB203580 concentration 196 ± 25.642 vs 492 ± 44.244)and the intestinal tissue level IL-8 and IFN-γwere notably lower in group A and group B(P < 0.01),

they were expressed much higher in group A campared with group D (P < 0.01). Campared group A and B, the serum level IL-8 and IFN-γand the intestinal tissue level IFN-γ were higher (P < 0.05), but the intestinal tissue level IL-8 and DAI scores were expressed no difference (P = 0.314, P = 0.139). Conclusion: There is therapeutic effect with Sb for UC, which MCE may be related to reduce serum and intestinal tissue level of IL-8 and IFN-γ. Key Word(s): 1. ulcerative colitis; 2. SASP; Presenting Author: CHANG QING YIN Corresponding Author: CHANG QING YIN Objective: To observe the expression and clinical significance of E-cadherin (E-CAD) and Matrix metalloproteinase-7 (MMP-7)in colorectal carcinoma. Methods: Expression of E-CAD and MMP-7 in 20 cases of normal colorectal mucosa, 34 cases of colorectal adenoma and 62 cases of colorectal carcinoma was determined by immunohistochemical staining, and relationship between E-CAD and MMP-7 expression and pathological features in 62 cases of colorectal carcinoma. Results: The positive expression of E-CAD in normal colorectal mucosa was significantly higher than those in adenoma and colorectal carcinoma. In colorectal carcinoma and adenoma, the positive expression of MMP-7 was significantly higher than those in normal colorectal mucosa. The expression of E-CAD was decreased and that of MMP-7 was increased with Dukes stage and depth of invasion rising and lymph node transferring.

Conclusion: Serrated polyps seem to be markers of advanced colonic neoplasia and synchronous cancer. These patients should be regarded as alternate accelerated pathway of colorectal neoplasia and undergo more frequent surveillance colonoscopies then previously thought. (1)  Rondagh et al. Endoscopy 2011; 43 Key Word(s): 1. Serrated Polyps; 2. Colorectal Neoplasia; 3. Serrated Polyposis; Presenting Author: SUNLI YING Corresponding Author: SUNLI YING Affiliations: The First Affiliated Hospital of Harbin Medical University Objective: To investigate the therapeutic effect of saccharomyces boulardii (Sb) in ulcerative colitis through observation of it’s serum level of IL-8 and

IFN-γin TNBS-induced Talazoparib rat colitis. Methods: forty male Wister rats (250–300 g) were randomized into four groups containing ten rats each, namely, colitis groups (group A and B and C), normal control group (group D).Then group A was given SB(800 mg/kg.d),group B was given SASP(200 mg/kg.d),while group C was given 1 ml normal saline for seven days. All of the rats were anesthetized, take blood and colon tissue. Expression of serum level IL-8 and IFN-γwere detected using ELISA, the intestinal tissue were detected by immunohistochemical staining. Results: Campared with group C, DAI scores(1.62 ± 0.73, 1.34 ± 0.605

vs 2.93 ± 0.752) and the serum level IL-8 and IFN-γ (536.32 ± 38.916, 400.38 ± 34.146 vs 783.05 ± 49.522; 328 ± 23.166, check details 196 ± 25.642 vs 492 ± 44.244)and the intestinal tissue level IL-8 and IFN-γwere notably lower in group A and group B(P < 0.01),

they were expressed much higher in group A campared with group D (P < 0.01). Campared group A and B, the serum level IL-8 and IFN-γand the intestinal tissue level IFN-γ were higher (P < 0.05), but the intestinal tissue level IL-8 and DAI scores were expressed no difference (P = 0.314, P = 0.139). Conclusion: There is therapeutic effect with Sb for UC, which MCE may be related to reduce serum and intestinal tissue level of IL-8 and IFN-γ. Key Word(s): 1. ulcerative colitis; 2. SASP; Presenting Author: CHANG QING YIN Corresponding Author: CHANG QING YIN Objective: To observe the expression and clinical significance of E-cadherin (E-CAD) and Matrix metalloproteinase-7 (MMP-7)in colorectal carcinoma. Methods: Expression of E-CAD and MMP-7 in 20 cases of normal colorectal mucosa, 34 cases of colorectal adenoma and 62 cases of colorectal carcinoma was determined by immunohistochemical staining, and relationship between E-CAD and MMP-7 expression and pathological features in 62 cases of colorectal carcinoma. Results: The positive expression of E-CAD in normal colorectal mucosa was significantly higher than those in adenoma and colorectal carcinoma. In colorectal carcinoma and adenoma, the positive expression of MMP-7 was significantly higher than those in normal colorectal mucosa. The expression of E-CAD was decreased and that of MMP-7 was increased with Dukes stage and depth of invasion rising and lymph node transferring.